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| Fontes,Aparecida Maria; Kashima,Simone; Bonfim-Silva,Ricardo; Azevedo,Rochele; Abraham,Kuruvilla Joseph; Albuquerque,Sérgio Roberto Lopes; Bordin,José Orlando; Langhi Júnior,Dante Mário; Covas,Dimas Tadeu. |
| Complement receptor 1 (CR1) gene polymorphisms that are associated with Knops blood group antigens may influence the binding of Plasmodium parasites to erythrocytes, thereby affecting susceptibility to malaria. The aim of this study was to evaluate the genotype and allele and haplotype frequencies of single-nucleotide polymorphisms (SNPs) of Knops blood group antigens and examine their association with susceptibility to malaria in an endemic area of Brazil. One hundred and twenty-six individuals from the Brazilian Amazon were studied. The CR1-genomic fragment was amplified by PCR and six SNPs and haplotypes were identified after DNA sequence analysis. Allele and haplotype frequencies revealed that the Kn b allele and H8 haplotype were possibly associated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Brazilian Amazon population; CR1 haplotypes; Knops blood group polymorphism; Malaria. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400002 |
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| Pinto,Mariana Tomazini; Malta,Tathiane Maistro; Rodrigues,Evandra Strazza; Takayanagui,Osvaldo Massaiti; Tanaka,Yuetsu; Covas,Dimas Tadeu; Kashima,Simone. |
| ABSTRACT Human T-lymphotropic virus type 1 (HTLV-1) is a human retrovirus related to the chronic neuroinflammatory disease HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). CD4+ T cells activation appears to play a key role on HTLV-1 infection. Here we investigated the expression of genes associated to T cell activation CD3e molecule, epsilon (CD3?), lymphocyte-specific protein tyrosine kinase (LCK), vav 1 guanine nucleotide exchange factor (VAV1), and zeta-chain (TCR) associated protein kinase 70 kDa (ZAP70) on T lymphocytes of HTLV-1-infected individuals and compared to healthy uninfected individuals (CT). We observed that CD3?, LCK, ZAP70, and VAV1 gene expression were increased in CD4+ T cells from HAM/TSP group compared to HTLV-1... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HTLV-1; HAM/TSP; CD4+ T cell activation; Gene expression. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000600578 |
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| Victoria,Marilú Barbieri; Victoria,Flamir da Silva; Torres,Kátia Luz; Kashima,Simone; Covas,Dimas Tadeu; Malheiro,Adriana. |
| The association of HIV infection and hepatitis C virus (HCV) infection often occurs because both viruses share the same transmission routes, increasing the possibility of HIV/HCV coinfection. World prevalence greater than 30% of coinfected cases is estimated, and it can reach 90% depending on the transmission route. With the aim of determining the frequency and profile of HIV/HCV coinfected patients, a descriptive analysis was carried out with patients with HIV/AIDS whose serology was positive for hepatitis C virus (HCV), cared for at the Fundação de Medicina Tropical do Amazonas from 2000 to 2007. In the present study, of the 2,653 AIDS cases notified in SINAN, 1,582 patients underwent serology test for hepatitis C, and a frequency of 4.42% (n = 70) of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coinfection; Hepatitis C; HIV; Epidemiology; Transmission. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200004 |
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| Slavov,Svetoslav Nanev; Kashima,Simone; Silva-Pinto,Ana Cristina; Amarilla,Alberto Anastacio; Aquino,Victor Hugo; Covas,Dimas Tadeu. |
| Human parvovirus B19 is a well-known cause of severe conditions in patients with sickle cell disease, but the molecular mechanisms of the infection are insufficiently understood. The different clinical outcome of the acute parvovirus B19 infection in two pediatric patients with sickle cell disease has been examined. One of them developed life-threatening condition requiring emergency transfusions, while the other had asymptomatic infection, diagnosed occasionally. Both cases had high viral load and identical subgenotype, indicating that the viral molecular characteristics play a minimal role in the infection outcome. |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Human parvovirus B19; Viral load; Aplastic crisis; Subgenotypes. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000100017 |
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| Gasparotto,Elainy Patricia Lino; Tognon,Raquel; Ferreira,Aline Fernanda; Oliveira,Gislane Lelis Vilela; Palma,Patrícia Vianna Bonini; Zanichelli,Maria Aparecida; Souto,Elizabeth Xisto; Velano,Carlos Eduardo Engel; Simões,Belinda Pinto; Carrara,Rita de Cassia Viu; Kashima,Simone; Covas,Dimas Tadeu; Castro,Fabíola Attie de; Souza,Ana Maria de. |
| Apoptosis deregulation might have a role in the pathophysiology of polycythemia vera (PV). This study evaluated Bcl-2 molecule expression in CD34+ cells and leukocytes in 12 PV patients. Gene expression was investigated by real time PCR using SybrGreen Quantitect kit and protein expression was evaluated by western-blotting. JAK2 V617F mutation was detected according to Baxter et al (2005). CD34+ cells from PV patients presented higher levels of A1 and Mcl-1 expression (median: 22.6 and 5.2, respectively) in comparison with controls (0.9 and 0.5, p=0.004 and p=0.020); while Bcl-2 and Bcl-xL expression decreased in PV patients (0.18 and 1.19) compared with controls (1.39 and 2.01, p=0.006 and p=0.020). CD34+ cells in PV patients showed an elevated Bid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polycythemia vera; Gene mutation; Gene expression; Apoptosis; Bcl-2 family members. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502011000400025 |
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