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アルファルファとVerticillium albo-atrumの相互作用 : 6. 菌体細胞壁成分に対して高いPAL活性を示す細胞系統の選抜とその特性 OAK
Koike, Masanori; Katsumata, Yukiyoshi; Amemiya, Yoshimiki; Shimada, Tohru.
To obtain alfalfa cell lines with high phenylalanine ammonia-lyase (PAL) activity in response to heat-released cell wall components (elicitor) of an alfalfa pathogen (Verticillium albo-atrum), in vitro selection was performed. The selection using a replica plating methods was carried out on 65 cell lines derived from suspension cultures treated and/or non-treated with N-metyl-N'-nitro-N-nitrosoguanidine (MNNG) of the susceptible genotype of cv. Vertus. Three stable high PAL cell lines in response to elicitor (two cell lines recovered from MNNG treatment, one from non-MNNG treatment) were isolated. All these cell lines showed higher PAL activities, lignin depositions and phytoalexin accumulations than the parental cell line after treatment with active V....
Palavras-chave: Alfalfa; In vitro selection; Phenylalanine ammonia-lyase; アルファルファ; 細胞選抜; Verticillium albo-atrum; フェニルアラニンアンモニアリアーゼ.
Ano: 1995 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1770
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