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	Provedor de dados Ciênc. Tecnol. Aliment. (1) BJM (1) Autor Kawaguti,Haroldo Yukio (2) Sato,Hélia Harumi (2) Fleuri,Luciana Francisco (1) Manrich,Eiric (1) Palavra-chave Isomaltulose (2) Calcium alginate (1) Erwinia sp. (1) Glucosyltransferase (1) Immobilized cells (1) Optimization (1) Response surface (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2005 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Production of glucosyltransferase by Erwinia sp. using experimental design and response surface methodology BJM Kawaguti,Haroldo Yukio; Manrich,Eiric; Fleuri,Luciana Francisco; Sato,Hélia Harumi. Glucosyltransferase produced by strain Erwinia sp. is an intracellular enzyme that catalyzes the formation of isomaltulose from sucrose. Isomaltulose is a non-cariogenic reducing dissacharide commercially used in foods. Response surface methodology and 2³-factorial central composite design were employed to optimize a fermentation medium for the production of glucosyltransferase by Erwinia sp. in shaken flasks at 200 rpm and 30ºC. The three variables involved in this study were sugar cane molasses (SCM), corn steep liquor (CSL) and yeast extract Prodex Lac SD (YEP). The statistical analysis of the results showed that, in the range studied, all the factors had a significant effect on glucosyltransferase production and the optimum medium composition for... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucosyltransferase; Isomaltulose; Optimization; Response surface. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300005 Production of isomaltulose obtained by Erwinia sp. cells submitted to different treatments and immobilized in calcium alginate Ciênc. Tecnol. Aliment. Kawaguti,Haroldo Yukio; Sato,Hélia Harumi. In recent decades, there has been an increase in the studies of isomaltulose obtainment, due to its physicochemical properties and physiological health benefits. These properties, which include low cariogenicity, low glycemic index and greater stability, allow the use of this sweetener as a substitute for sucrose in foods; besides the fact that it can be converted to isomalt, a dietary non-cariogenic sugar alcohol used in pharmaceuticals as well as in the food industry. Isomaltulose (6-O-α-D-glucopyronosyl-1-6-D-fructofuranose) is a disaccharide reducer obtained by the enzymatic conversion of sucrose - the α-glucosyltransferase enzyme. Different treatments were performed for the preparation of whole cells; lysed cells; and crude enzyme extract of Erwinia... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calcium alginate; Immobilized cells; Erwinia sp.; Isomaltulose. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100039 Registros recuperados: 2 Primeira ... 1 ... Última

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