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Genetic approaches for studying transgene inheritance and genetic recombination in three successive generations of transformed tobacco Genet. Mol. Biol.
Tizaoui,Kalthoum; Kchouk,Mohamed Elyes.
Transgene integration into plant genomes is a complex process accompanied by molecular rearrangements. Classic methods that are normally used to study transgenic population genetics are generally inadequate for assessing such integration. Two major characteristics of transgenic populations are that a transgenic genome may harbor many copies of the transgene and that molecular rearrangements can create an unstable transgenic locus. In this work, we examined the segregation of T1, T2 and T3 transgenic tobacco progenies. Since transfer DNA (T-DNA) contains the NptII selectable marker gene that confers resistance to kanamycin, we used this characteristic in developing a method to estimate the number of functional inserts integrated into the genome. This...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Copy number variation; Gene rearrangements; Genetic inheritance; Transgenic.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000400015
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