|
|
|
|
|
| Schroeder,Regina Barbosa; Michelon,Tatiana Ferreira; Garbin,Gabriela; Garcia,Valter; Silveira,Janaina Gomes da; Santos,Luciano; Neumann,Jorge; Keitel,Elizete. |
| Human herpesvirus type 6-(HHV-6) has been associated with morbidity after liver transplantation. OBJECTIVE: The aim of this study was to determine the HHV-6 seroprevalence among donor-recipient pairs, analyze the incidence of early active infection, its clinical manifestation, interaction with CMV, and the related morbidity in the first year after kidney transplantation. METHODS: 46 donor-recipient pairs had IgG evaluated by ELISA before transplantation: HHV-6(Pambio - USA) and CMV-(Roche - USA). A frozen whole blood sample collected weekly (from the 1st to the 6th week) was retrospectively tested for HHV-6 viral load (VL) determination by real time quantitative PCR (qPCR, Nanogen - Italy). Patients were preemptively surveyed for CMV by pp65 antigenemia... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytomegalovirus infections; Herpesvirus 6/human; DNA virus infections; Clinical diagnosis; Polymerase chain reaction. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200007 |
| |
|
|
| Montagner,Juliana; Michelon,Tatiana; Fontanelle,Barbara; Oliveira,Alexandre; Silveira,Janaina; Schroeder,Regina; Neumann,Jorge; Keitel,Elizete; Alexandre,Claudio Osmar Pereira. |
| INTRODUCTION: BKV nephropathy (BKN) causes kidney graft loss, whose specific diagnosis is invasive and might be predicted by the early detection of active viral infection. OBJECTIVE: Determine the BKV-infection prevalence in late kidney graft dysfunction by urinary decoy cell (DC) and viral DNA detection in urine (viruria) and blood (viremia; active infection). METHODS: Kidney recipients with >1 month follow-up and creatinine >1.5 mg/dL and/or recent increasing >20% (n = 120) had their urine and blood tested for BKV by semi-nested PCR, DC searching, and graft biopsy. PCR-positive patients were classified as 1+, 2+, 3+. DC, viruria and viremia prevalence, sensitivity, specificity, and likelihood ratio (LR) were determined (Table 2x2). Diagnosis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BK virus; Decoy cells; Kidney transplantation; PCR; Viremia; Viruria. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000200010 |
| |
|
|
|
