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Total and active microbial communities and phoD as affected by phosphate depletion and pH in soil Organic Eprints
Ragot, Sabine A.; Huguenin-Elie, Olivier; Kertesz, Michael A.; Frossard, Emmanuel; Bünemann, Else K..
Background and Aims Soil microbial communities contribute to organic phosphorus cycling in a variety of ways, including secretion of the PhoD alkaline phosphatase. We sampled a long-term grassland fertilization trial in Switzerland characterized by a natural pH gradient. We examined the effects of phosphate depletion and pH on total and active microbial community structures and on the structure and composition of the total and active phoD-harboring community. Methods Archaeal, bacterial and fungal communities were investigated using T-RFLP and phoD-harboring members of these communities were identified by 454-sequencing. Results Phosphate depletion decreased total, resin-extractable and organic phosphorus and changed the structure of all active...
Tipo: Journal paper Palavras-chave: Soil quality.
Ano: 2016 URL: http://orgprints.org/32321/1/ragot-etal-2017-PlantSoil-Vol408-Issue1-2-p15-30.pdf
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Soil phoD and phoX alkaline phosphatase gene diversity responds to multiple environmental factors Organic Eprints
Ragot, Sabine A.; Kertesz, Michael A.; Mészáros, Éva; Frossard, Emmanuel; Bünemann, Else K..
Alkaline phosphatases such as PhoD and PhoX are important in organic phosphorus cycling in soil. We identified the key organisms harboring the phoD and phoX genes in soil and explored the relationships between environmental factors and the phoD- and phoX-harboring community structures across three land uses located in arid to temperate climates on two continents using 454-sequencing. phoD was investigated using recently published primers, and new primers were designed to study phoX in soil. phoD was found in 1 archaeal, 13 bacterial and 2 fungal phyla, and phoX in 1 archaeal and 16 bacterial phyla. Dominant phoD-harboring phyla were Actinobacteria, Cyanobacteria, Deinococcus-Thermus, Firmicutes, Gemmatimonadetes, Planctomycetes and Proteobacteria, while...
Tipo: Journal paper Palavras-chave: Soil quality; Environmental aspects.
Ano: 2017 URL: http://orgprints.org/32317/1/ragot-etal-2017-FEMS-Vol93-Issue1-fiw212.pdf
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