|
|
|
|
|
| Khatoon,Lubna; Khan,Inam Ullah; Shah,Shahid Ali; Jan,Muhammad Ishtiaq; Ullah,Farhat; Malik,Salman Akbar. |
| Malaria is one of the serious diseases threatening human health in Pakistan and contributes to a large proportion of the total malaria deaths in South Asia. However, little is known about the nature and extent of genetic diversity of the malarial parasites circulating in Pakistan. This study was designed to assess the infection status of Plasmodium and the genetic diversity of Plasmodium vivax and Plasmodium falciparum by analyzing msp-3α, msp-3β and msp-1, msp-2 genes respectively using allele specific nested PCR and RFLP assays. For this purpose, 130 field isolates were collected from the individuals who exhibited clinical symptoms associated with malaria in the Kohat region of Khyber Pakhtoonkhwa (KPK), Pakistan. Among 130 blood samples collected, P.... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Plasmodium vivax; Plasmodium falciparum; Genetic variation; Pakistan. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000200014 |
| |
|
|
| Khatoon,Lubna; Baliraine,Frederick N.; Malik,Salman A.; Yan,Guiyun. |
| Plasmodium vivax and Plasmodium falciparum are becoming resistant to drugs including antifolates, sulphonamides and chloroquine. This study was focused at sequence analysis of resistant genes of these parasites against sulphadoxine-pyrimethamine and chloroquine, from Bannu, Pakistan. Known mutations were detected at codons 57, 58 and 117 of pvdhfr gene of P. vivax, while none of the isolates had any pvdhps mutation. Similarly P. falciparum isolates exhibited double 59R + 108N mutations in pfdhfr, and single 437G in pfdhps thus demonstrating the existance of triple mutant 59R + 108N + 437G haplotype in this region. The key chloroquine resistance mutation, 76T in pfcrt was observed in 100% of the P. falciparum isolates, with haplotype SVMNT which is also... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Plasmodium vivax; Plasmodium falciparum; Sequence analysis; Pakistan. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000500013 |
| |
|
|
|
