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Evaluation of cytochrome P-450 concentration in Saccharomyces cerevisiae strains BJPS
Matuo,Míriam Cristina Sakuragui; Kikuchi,Irene Satiko; Pinto,Terezinha de Jesus Andreoli.
Saccharomyces cerevisiae has been widely used in mutagenicity tests due to the presence of a cytochrome P-450 system, capable of metabolizing promutagens to active mutagens. There are a large number of S. cerevisiae strains with varying abilities to produce cytochrome P-450. However, strain selection and ideal cultivation conditions are not well defined. We compared cytochrome P-450 levels in four different S. cerevisiae strains and evaluated the cultivation conditions necessary to obtain the highest levels. The amount of cytochrome P-450 produced by each strain varied, as did the incubation time needed to reach the maximum level. The highest cytochrome P-450 concentrations were found in media containing fermentable sugars. The NCYC 240 strain produced the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharomyces cerevisiae; Cytochrome P-450/obtention; Cytochrome P-450/cultivation conditions; Ethanol; Carbon sources.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300011
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