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Establishment of a pheasant (Phasianus colchicus) spermatogonial stem cell line for the production of interspecies germ line chimeras Electron. J. Biotechnol.
Kim,Jeong Hyun; Sharma,Neelesh; Kim,Sung Woo; Sodhi,Simrinder Singh; Ghosh,Mrinmoy; Kim,Nam Eun; Mongre,Raj Kumar; Oh,Sung Jong; Jeong,Dong Kee.
Background Spermatogonial stem cells (SSCs) are important for the production of interspecies germ line chimeras. The interspecies germ cell transfer technique has been suggested as a way to conserve endangered birds. Our objective was to develop a technique for restoring endangered birds by developing interspecies germ line chimeras between pheasant (Phasianus colchicus) and chicken (Gallus gallus) with SSCs. Results SSCs were isolated from the surgically removed testis of a pheasant. Growth conditions for pheasant SSCs were established by co-culturing STO (SIM mouse embryo-derived thioguanine and ouabain resistant) cells and pheasant SSCs. The colony-forming cells divided and proliferated stably to yield an established SSC line. Pheasant SSCs showed...
Tipo: Journal article Palavras-chave: Endangered birds; In vitro culture; Recipient chicken embryos; SSC transplantation.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500004
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Recent advances in sow nutrition R. Bras. Zootec.
Kim,Sung Woo.
Sows produce a larger number of fetuses than before and these fetuses possess genetic potentials to grow faster than before. Recent comparison shows that a porcine fetus is 40% heavier than 40 years ago. Thus the nutritional management of sows has been updated to reflect these genetic changes. Our recent study quantified nutritional needs for sows to support the growth of fetuses and mammary glands during gestation. Amino acid needs for fetal growth and mammary growth during late gestation (d 70 to farrowing) significantly greater than those needs during early gestation (until d 70). Considering these increases, daily requirement of true ileal digestible Lys for a primiparous sows increases from 7 g (until d 70) to 15 g (d 70 to farrowing). Required...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Amino acids; Fetus; Gestation; Lactation; Oxidative stress; Sows.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300033
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