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Non-destructive in vitro selection of microspore-derived embryos with the fertility restorer gene for CMS Ogu-INRA in winter oilseed rape (Brassica napus L.) Electron. J. Biotechnol.
Havlíčková,Lenka; Klíma,Miroslav; Přibylová,Marie; Hilgert-Delgado,Alois Antonín; Kučera,Vratislav; Čurn,Vladislav.
Background Microspore embryogenesis and cytoplasmic male sterility system (CMS) are two approaches widely exploited in Brassica napus breeding for production of homozygous doubled haploid (DH) lines and F1 hybrids respectively. Cytoplasmic male sterility system (CMS) is one of the most important pollination systems for hybrid seed production and utilisation of doubled haploid system to quickly prepare fully homozygous fertility restorer lines for CMS Ogu-INRA is very beneficial. Generally, only a small part of microspore-derived embryos is used for plant regeneration, without any knowledge about their properties. Therefore, the possibility of early detection of desirable genotypes bearing a single dominant nuclear fertility restorer (Rfo) gene, can double...
Tipo: Journal article Palavras-chave: Cytoplasmic male sterility; Microspore embryogenesis; Ogura CMS; Oilseed rape; Rfo gene.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100011
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Detection of self-incompatible oilseed rape plants (Brassica napus L.) based on molecular markers for identification of the class I S haplotype Genet. Mol. Biol.
Havlícková,Lenka; Jozová,Eva; Klíma,Miroslav; Kucera,Vratislav; Curn,Vladislav.
The selection of desirable genotypes with recessive characteristics, such as self-incompatible plants, is often difficult or even impossible and represents a crucial barrier in accelerating the breeding process. Molecular approaches and selection based on molecular markers can allow breeders to overcome this limitation. The use of self-incompatibility is an alternative in hybrid breeding of oilseed rape. Unfortunately, stable self-incompatibility is recessive and phenotype-based selection is very difficult and time-consuming. The development of reliable molecular markers for detecting desirable plants with functional self-incompatible genes is of great importance for breeders and allows selection at early stages of plant growth. Because most of these...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Double haploid; Hybrid breeding; MAS; S locus; SLG.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400012
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