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| Lüdtke,Charli Beatriz; Coutinho,Mariana Loner; Jouglard,Sandra Denise Dorneles; Moreira,Cecília Nunes; Fernandes,Cláudia Hartleben Pinho; Brod,Claudiomar Soares; Haake,David A.; Ko,Albert Icksang; Dellagostin,Odir Antonio; Aleixo,José Antonio Guimarães. |
| Two hybridomas secreting monoclonal antibodies (MAbs) that react with a lipoprotein (LipL32) of the outer membrane of pathogenic Leptospira were obtained. For hybridoma production, spleen cells from BALB/c mice imunized with recombinant LipL32 (rLipL32) were fused to SP2/O-Ag14 cells, selected in HAT medium and screened in an indirect ELISA. One MAb produced was of the IgG2b isotype and the other was an IgM. MAbs specificity was confirmed by indirect ELISA and immunoblotting using purified rLipL32 and whole-cell antigen preparations from Escherichia coli (E. coli) expressing LipL32 and from pathogenic and non-pathogenic serovars. Both Mabs reacted with most of the pathogenic serovars tested and none reacted with non-pathogenic Leptospira. The MAbs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Monoclonal antibodies; LipL32. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500001 |
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| Tomich,Renata Graça Pinto; Bomfim,Maria Rosa Quaresma; Koury,Matilde Cota; Pellegrin,Aiesca Oliveira; Pellegrin,Luiz Alberto; Ko,Albert Icksang; Barbosa-Stancioli,Edel Figueiredo. |
| This investigation was carried out in Brazilian Pantanal: region with important biodiversity. This region's climatic conditions, hydrology and geomorphology as well as the existence of great variety of wild species favor the maintenance of the Leptospira in the environment. The aim of this study was to evaluate IgG ELISA with recombinant protein LipL32 in comparison with microscopic agglutination test (MAT) and additionally contribute to the knowledge of the distribution of the one of most important worldwide zoonotic infection, assessing the seropositivity of bovine leptospirosis in beef cattle herds of Brazilian Pantanal, an important ecological preserved area, where cattle constitute not only the most important economic resource but also the major... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine Leptospirosis; IgG ELISA rLipL32; MAT. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400017 |
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