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Mitochondrial DNA synthesis studied autoradiographically in various cell types in vivo BJMBR
Korr,H.; Kurz,C.; Seidler,T.O.; Sommer,D.; Schmitz,C..
It is generally accepted that mitochondria are able to proliferate even in postmitotic cells due to their natural turnover and also to satisfy increased cell energy requirements. However, no detailed studies are available, particularly with respect to specific cell types. Since [3H]-thymidine is incorporated not only into nuclear (n) DNA but also into the DNA of cytoplasmic mitochondria, an autoradiographic approach was developed at the light microscopy level in order to study basic questions of mitochondrial (mt) proliferation in organs of rodents in situ via the cytoplasmic incorporation of [3H]-thymidine injected into the animals 1 h before sacrifice. Experiments carried out on mice after X-irradiation showed that cytoplasmic labeling was not due to a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aging; Autoradiography; Mitochondrial DNA synthesis; Turnover of mitochondria; Unscheduled DNA synthesis.
Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000200012
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