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| Pescador,Rosete; Kerbauy,Gilberto Barbante; Viviani,Daniela; Kraus,Jane Elizabeth. |
| Somatic embryogenesis represents a valuable tool for the studies on the basic aspects of plant embryo development. Today this process is used as a potencial technique for large-scale plant micropropagation although, so far, it has been applied to only a small number of species. However, when somatic embryos are malformed they are considered economically useless. In Acca sellowiana (O. Berg) Burret, an important fruit-producing crop, large amounts of anomalous somatic embryos (76.3%) were found just after 40 days of culture of explants in a 2,4-D containing medium. Among the anomalous forms found in the cotiledonary stage, 12.2% consisted of fused embryos, 40.4% displayed fused cotyledons, 13.0% presented supernumerary cotyledons, and 10.7% showed absence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 2; 4-D feijoa morphohistological analysis somaclonal variation somatic embryogenesis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042008000100014 |
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| Pescador,Rosete; Kerbauy,Gilberto Barbante; Strassburg,Rosali Constantino; Kraus,Jane Elizabeth. |
| Acca sellowiana has anatropous, bitegmic and crassinucellate ovules. The outer and inner integuments are double-layered except in the micropyle, where they are composed of more layers; the micropyle is zig-zag shaped. The egg apparatus lies at the micropylar pole, and the zynergids present a conspicuous filiform apparatus. The antipodal cells are present in the chalazal region, persisting before the occurrence of double fertilization. The zygote is visible 21 days after pollination; nuclear endosperm is already present. The first mitotic division of the zygote occurs at 24th day. The globular, cordiform and torpedo embryo stages can be seen at 30, 45 and 60 days after pollination, respectively. The mature embryo characterized by the presence of a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Feijoa; Megagametophyte; Ovule; Pineapple guava; Zygotic embryo. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000100017 |
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| Mercier,Helenice; Souza,Beatriz Maia; Kraus,Jane Elizabeth; Hamasaki,Regina Mayumi; Sotta,Bruno. |
| The in vitro culture of pineapple leaves on a shoot induction medium (SIM) results in the formation of protuberances and further development in shoots, and plantlets. The contents of endogenous indoleacetic acid (IAA) and five cytokinins (Cks), N6(2-isopentenyl)adenine (iP), N6(2-isopentenyl)adenosine (iPR), zeatin (Z), zeatin riboside (ZR) and N6-benzyladenine (BA), present in the basal portion of those leaves, were correlated to the organogenic response that occurs over 15 days of culture. The endogenous auxin/cytokinins ratio was lowest on the 3rd day, mainly due to a strong increase in the iP level. It seems that endogenous iP concentration triggered the induction signal for an organogenic response in pineapple leaf bases. The rise in iP content... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ananas comosus; Auxin; Bromeliaceae; Cytokinin; Hormone signalling; Plant regeneration. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000200006 |
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