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Array-CGH testing in spontaneous abortions with normal karyotypes Genet. Mol. Biol.
Borovik,Cleide L.; Perez,Ana Beatriz A.; Silva,Luciana R.J. da; Krepischi-Santos,Ana Cristina V.; Costa,Silvia S.; Rosenberg,Carla.
In about 50% of first trimester spontaneous abortion the cause remains undetermined after standard cytogenetic investigation. We evaluated the usefulness of array-CGH in diagnosing chromosome abnormalities in products of conception from first trimester spontaneous abortions. Cell culture was carried out in short- and long-term cultures of 54 specimens and cytogenetic analysis was successful in 49 of them. Cytogenetic abnormalities (numerical and structural) were detected in 22 (44.89%) specimens. Subsequent, array-CGH based on large insert clones spaced at ~1 Mb intervals over the whole genome was used in 17 cases with normal G-banding karyotype. This revealed chromosome aneuplodies in three additional cases, giving a final total of 51% cases in which an...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spontaneous abortion; Chromosomal aberrations; Array-CGH.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300004
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