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Somatic embryogenesis and plant regeneration from zygotic embryo explants of onion Horticultura Brasileira
Sivanesan,Iyyakkannu; Kyoung,Kim E; Kyoung,Kim M; Young,Ko E; Park,Se W.
ABSTRACT: The study was undertaken to examine the effect of two synthetic auxins on somatic embryogenesis and plant regeneration from mature embryo explants of 16 onion cultivars. Cotyledons were removed from the embryos and remaining portions were cultured on MS medium supplemented with 1.0 mg/L 2,4-D and 5.0 mg/L picloram alone or in combination (1.0 mg/L 2,4-D + 2.5 mg/L picloram) to produce embryogenic callus. MS medium supplemented with 5.0 mg/L picloram was found to be the best one for both embryogenic callus induction (85%) and callus diameter (3.8 mm). Of the 16 cultivars studied, Yeoeuijuhwang exhibited the lowest frequency of embryogenic callus induction (50.5%), whereas all the other 15 cultivars showed more than 60% embryogenic callus...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa; Auxin; Embryogenic callus; In vitro propagation; Picloram..
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362015000400441
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