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ESTUDIO DE LA EXPRESIÓN DE GENES QUE CODIFICAN PARA PUTATIVAS PROTEÍNAS PR EN YUCA (Manihot esculenta Crantz) Acta biol.Colomb.
HERRERA,Mariana; PORTILLO,David; PULIDO,Marlon Adrian; DÍAZ TATIS,Paula Alejandra; LÓPEZ CARRASCAL,Camilo Ernesto.
RESUMEN Posterior al reconocimiento de agentes patógenos las plantas activan una serie de cascadas de señalización que culminan con la activación de factores de transcripción. Esto genera una concomitante reprogramación de la expresión génica que incluye la activación de la transcripción de los genes PR (relacionados con patogenicidad). Las proteínas PR son conocidas por poseer actividad antimicrobiana y evitan la posterior colonización del patógeno. En este estudio se empleó una aproximación bioinformática para identificar el repertorio de posibles proteínas PR en el genoma de yuca. Adicionalmente, se evaluó la expresión de nueve genes PR a lo largo del tiempo en variedades de yuca resistentes y susceptibles en respuesta a la inoculación con la bacteria...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacteriosis vascular; Expresión génica; Genes relacionados con patogenicidad; Xanthomonas axonopodis pv. manihotis.
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000300242
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COMPARING INOCULATION METHODS TO EVALUATE THE GROWTH OF Xanthomonas axonopodis pv. manihotis ON CASSAVA PLANTS Acta biol.Colomb.
MUÑOZ BODNAR,Alejandra; CRUZ GÓMEZ,Leidy Mariel; BERNAL,Adriana; SZUREK,Boris; LÓPEZ CARRASCAL,Camilo Ernesto.
Xanthomonas axonopodis pv. manihotis (Xam) is the causal agent of cassava bacterial blight (CBB), a major disease for cassava crops in South America and Africa. Until now the development of the disease is measured via AUDPC (Area Under Disease Progress Curve) but no reliable quantitative methods are available probably due to high variability of bacterial growth in planta. To establish an accurate method for bacterial quantification during the course of Xam infection within the host tissues, we analyzed bacterial populations upon stem and leaf-puncturing as well as leaf-clipping of cassava varieties MCOL1522 and SG107-35 challenged with the virulent Xam strain CIO151. Here, we show that the movement of bacteria along the tissues and especially in leaves is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial growth; Cassava; Inoculation methods.
Ano: 2015 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2015000200005
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QTL IDENTIFICATION FOR CASSAVA BACTERIAL BLIGHT RESISTANCE UNDER NATURAL INFECTION CONDITIONS Acta biol.Colomb.
SOTO SEDANO,Johana; MORA MORENO,Rubén Eduardo; CALLE,Fernando; LÓPEZ CARRASCAL,Camilo Ernesto.
Cassava, Manihot esculenta Crantz, represents the main food source for more than one billion people. Cassava's production is affected by several diseases, one of the most serious is cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis (Xam). A quantitative trait loci (QTL) analysis for CBB resistance was performed under natural infection conditions, using a mapping population of 99 full-sibs genotypes highly segregant and a SNP-based high dense genetic map. The phenotypic evaluation was carried out in Puerto López, Meta, Colombia, during the rainy season in 2015. Both resistant and susceptible transgressive segregants were detected in the mapping population. Through a non-parametric interval mapping analysis, two QTL were detected,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cassava; Molecular marker; QTL; Resistance; SNPs; Xanthomonas axonopodis pv. manihotis.
Ano: 2017 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2017000100002
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UNRAVELING THE MOLECULES HIDDEN IN THE GRAY SHADOWS OF QUANTITATIVE DISEASE RESISTANCE TO PATHOGENS Acta biol.Colomb.
VÁSQUEZ,Andrea Ximena; SOTO SEDANO,Johana Carolina; LÓPEZ CARRASCAL,Camilo Ernesto.
ABSTRACT One of the most challenging questions in plant breeding and molecular plant pathology research is what are the genetic and molecular bases of quantitative disease resistance (QDR)?. The scarce knowledge of how this type of resistance works has hindered plant breeders to fully take advantage of it. To overcome these obstacles new methodologies for the study of quantitative traits have been developed. Approaches such as genetic mapping, identification of quantitative trait loci (QTL) and association mapping, including candidate gene approach and genome wide association studies, have been historically undertaken to dissect quantitative traits and therefore to study QDR. Additionally, great advances in quantitative phenotypic data collection have been...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breeding; Complex traits; Genome; Gene expression; Plant immunity; Quantitative disease resistance (QDR); Quantitative trait loci (QTL).
Ano: 2018 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000100005
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IDENTIFICACIÓN DE POLIMORFISMOS EN EL GEN RXAM1 DE YUCA Y SU ASOCIACIÓN CON LA RESISTENCIA A LA BACTERIOSIS VASCULAR Acta biol.Colomb.
BARRERA,Andrea del Pilar; SOTO-SEDANO,Johana; LÓPEZ CARRASCAL,Camilo Ernesto.
RESUMEN La yuca (Manihot esculenta Crantz) es un cultivo importante en regiones del trópico que proporciona alimento para cerca de 1000 millones de personas en todo el mundo. La enfermedad bacteriana más importante es la bacteriosis vascular causada por Xanthomonas axonopodis pv. manihotis (Xam). Recientemente se logró identificar un gen de resistencia denominado RXAM1, el cual codifica para una proteína que posee un dominio LRR (Leucine-Rich Repeat) extracelular y un dominio STK (Serine Threonine Kinase) citoplasmático. RRXAM1 colocaliza con un QTL que explica el 13 % de la resistencia a la cepa CIO136 de Xam. En este trabajo se evaluó la respuesta a la infección con la cepa XamCIO136 en diez diferentes variedades de yuca lo cual permitió identificar que...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alelo; Diversidad; Receptor; Síntomas de plantas.
Ano: 2020 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200185
Registros recuperados: 5
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