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| TORRES,RODRIGO; MANRIQUEZ,PATRICIO H; DUARTE,CRISTIAN; NAVARRO,JORGE M; LAGOS,NELSON A; VARGAS,CRISTIAN A; LARDIES,MARCO A. |
| The assessment of the effects of Ocean Acidification (OA) on marine life has received increasing attention in recent marine research. On a mesocosmic scale, the CO2 levels in seawater can be manipulated to evaluate experimentally the consequences of OA on marine organisms (vertebrates and invertebrates). An ideal manipulation of carbonate chemistry should mimic exactly the changes to carbonate chemistry, which will occur in years to come. Although some methods have been described in the literature, here we describe in detail a simple, robust and inexpensive system to produce CO2-enriched seawater by bubbling the seawater with air-CO2 mixtures. The system uses mass flow controllers (MFC) to blend atmospheric air with pure CO2 to produce two pCO2 levels. The... |
| Tipo: Journal article |
Palavras-chave: Carbonate chemistry manipulation; Ocean acidification. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2013000400006 |
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