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Optimization of ultrasound-assisted extraction of procyanidins from perilla seed hull and their antioxidant activities in vitro 116
LI,Hui-Zhen; TAN,Yong-Lan; ZHANG,Zhi-Jun; XIA,Yao-Yao; LI,Xiao-Jun; CUI,Li-Xia; CHEN,Tie.
Abstract Ultrasound-assisted extraction (UAE) was used to extract procyanidins (PC) from the perilla seed hull. Four independent variables, namely, liquid-to-solid ratio, ethanol concentration, UAE time, and UAE temperature were investigated. Response surface methodology was performed on the basis of results of single-factor tests. The optimal conditions for PC extraction were as follows: a liquid-to-solid ratio of 15:1, an ethanol concentration of 62%, a UAE time of 29 min, and a UAE temperature of 53 °C. Under these conditions, the experimental yield of PC was 2.185 mg/g (n = 3), which is correlated with the predicted value of 2.2 mg/g. The extracted and purified PC showed excellent antioxidant activity against 2,2-diphenyl-1-picrylhydrazyl, hydroxyl,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Perilla seed hull; Ultrasound-assisted extraction; Procyanidins; Antioxidant activities.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000200378
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Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants and rosmarinic acid from perilla leaves using response surface methodology 116
LI,Hui-Zhen; ZHANG,Zhi-Jun; XUE,Jiao; CUI,Li-Xia; HOU,Tian-yu; LI,Xiao-Jun; CHEN,Tie.
Abstract Response surface methodology (RSM) was used to optimize ultrasound-assisted extraction (UAE) of functional components from perilla leaves. The factors investigated were ethanol concentration, extraction temperature, and extraction time. The results revealed that ethanol concentration had significant effects on all extraction parameters. Based on the RSM results, the optimal conditions were an ethanol concentration of 56%, a UAE temperature of 54 °C, and a UAE time of 55 min. Under these conditions, the experimental TPC (total phenolic content), RA (rosmarinic acid), FRAP (ferric reducing antioxidant power) and DPPH (1,1-diphenyl-2-picrylhydrazyl) values were 48.85 mg GAE/g DW (mg gallic acid equivalent /g of dry weight), 31.02 mg/g DW, 85.55 μmol...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Perilla leaves; Ultrasound-assisted extraction; Phenolic compounds; Antioxidants; Rosmarinic acid.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612016000400686
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