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LI,Li; GONG,Xiaoyan; REN,Hankun; WANG,Xiaoyue; HE,Yifan; DONG,Yinmao. |
Abstract Rice bean (Vigna umbellata L.) sprouts are richer in metabolites and biological activities after germination. The total polyphenol and total flavonoid contents were analyzed during the sprouting process. The antioxidant activities of the sprouts were tested using 1,1-diphenyl-2- picrylhydrazyl radical (DPPH) screening assay. To determine the reason for increased activity, quantitative analysis of 8 flavonoids, in the germination process of methyl jasmonate (MeJA)-treated and control groups, was performed using high-performance liquid chromatography coupled with diode array detection (HPLC/DAD) and partial least squares discriminant analysis (PLS/DA). The metabolites in MeJA-treated beans shows that addition of 100.0 μmol MeJA significantly... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rice bean; Methyl Jasmonate; Germination; Polyphenols; Antioxidant. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000500098 |
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LI,Li; DONG,Yinmao; REN,Hankun; XUE,Yan; MENG,Hong; LI,Minhui. |
Abstract Mung bean sprouts are a popular health food both in China and worldwide. We determined the optimal concentration of exogenous methyl jasmonate (MeJA) for the promotion of the sprouting in mung beans (Vigna radiata). The 1,1-diphenyl-2- picrylhydrazyl radical (DPPH) scavenging test showed that MeJA application resulted in significantly improved antioxidant capacity in the sprouts 72 h later. Measurement of total polyphenols in MeJA-treated beans from 0 to 168 h, using Folin–Ciocalteu colorimetry, showed that the polyphenols changing was significantly correlated with antioxidant activity. The main polyphenols isovitexin, kaempferol-3-O-rutinoside, daidzein, genistein, isoquercitrin, p-coumaric acid, and caffeic acid were quantified using... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mung beans sprouts; Methyl jasmonate; Antioxidants; Polyphenols; Metabolite pathway. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612017000300411 |
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WANG,Xiaoyue; GONG,Xiaoyan; ZHANG,Huina; ZHU,Wanshan; JIANG,Zewu; SHI,Yujing; LI,Li. |
Abstract To determine in vitro anti-aging activity and identify the active chemical constituents in Ginkgo leaf extracts. The antioxidant properties of Ginkgo biloba leaves extracts were evaluated using 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) scavenging and 2,2’-azino-bis (3-ethylbenzothiazoline)-6 sulfonic acid radical (ABTS) scavenging assays. The inhibitory effects of extracts and chemical constituents on reactive oxygen species, matrix metalloproteinase-1 (MMP-1) and collagen tpye I level were tested using human dermal fibroblasts (HDFs). Reverse-phase liquid chromatography (RPLC) and ultra-performance liquid chromatography tandem mass spectroscopy (UPLC/MS/MS) were used to quantitatively analyze flavonoids and terpene trilactones in the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ginkgo leaf; Antioxidant; Matrix metalloproteinase-1; Collagen I; Chemical analysis. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612020000200476 |
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LI,Li; ZHU,Wenyi; YANG,Jingxiu; LIU,Xiaolan; DONG,Yinmao. |
Abstract Objective This study was to establish a rapid quantitative method for determination of six characteristic flavonoids, including licochalcone A (1), liquiritigenin (2), glabridin (3), isoliquiritigenin (4), liquiritoside (5), and isoliquiritoside (6) in licorice by a ultra-performance convergence chromatography (UPC2) apparatus equipped with a photodiode-array detector (PDA). Methods: The flavonoids studied were separated on an ACQUITY UPC2 TM Torus 2-PIC column (2.1 × 100 mm, 1.7 μm) using gradient elution (85:15–70:30) with a mobile phase consisting of CO2 and methanol: formic acid (99.8:0.2, v/v), back pressure of 2,000 psi, flow rate of 0.8 mL/min, and detection at 254 nm. This method was further applied for the analysis of the flavonoids... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Licorice; UPC2/PDA; Quantify; Flavonoids. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612019000600426 |
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