SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Cancer cell inhibitors, named Atroporin and Kaotree, having molecular weights of 35 kDa and 6 kDa have been isolated from the venoms of Crotalus atrox and Naja naja kaouthia, respectively, by fractionation on high pressure liquid chromatography. The purified Atroporin and Kaotree showed killing effects on various types of human (breast, colon, liver, ovary, etc.) and animal cancer cells in concentrations as low as 0.5µg/ml, and having no effect on normal mouse kidney, liver, spleen, and erythrocytes up to 5.0µg/ml. Both Atroporin and Kaotree prevent the formation of ascitic tumors caused by myeloma cells in Balb/C mice. In addition, both Atroporin and Kaotree showed regression of ascitic tumors formed by myeloma cells. Atroporin and Kaotree complement each...

ANTI-LETHAL FACTOR FROM OPOSSUM SERUM IS A POTENT ANTIDOTE FOR ANIMAL, PLANT AND BACTERIAL TOXINS

Palavras-chave: Cancer; Biotherapy; Venom proteins.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000200005

Currently, the use of antivenoms is the only available treatment for envenomation caused by venomous animals namely, snake, scorpion, spider, tick and jelly fish. Antivenoms are generally produced in large animals, mostly in horses. A large percentage of the population is allergic to horse proteins. Several animals are known to be resistant to snakebites and the antihemorrhagic and anti-lethal components have been isolated from sera of opossum, mongoose, meerkat and hedgehog, as well as from venomous and non-venomous snakes. Anti-lethal factor named Lethal Toxin Neutralizing Factor (LTNF) has been isolated in purity from opossum (Didelphis virginiana) serum by high pressure liquid chromatography (HPLC). The molecular weight of LTNF is 63 kDa, and it does...

Sub-lethal injection of honeybee venom decreased the levels of endogenously present substances in organs of mice

Palavras-chave: Opossum; Didelphis virginiana; Lethal toxin neutralizing factor.

Ano: 1999

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301999000100005

Pharmacological substances such as adenosine deaminase (ADA), collagen, histamine, IgE, myoglobin, and nerve growth factor (NGF) are endogenously present in animals. Research from this laboratory reported decreased levels of ADA, histamine, IgE, and NGF in organs of mice injected with sub-lethal doses of cobra venom. The goal of this research is to observe the levels of ADA, collagen, histamine, IgE, myoglobin, and NGF in certain organs of mice injected with venom from the bee Apis mellifera. Adult Balb/c female mice IM injected with half lethal dose of bee venom were sacrificed after 2 and 8 hours for removal of organs. The homogenates of the organs were assayed by ELISA for ADA, collagen, histamine, IgE, myoglobin, and NGF using respective antisera....

SUB-LETHAL INJECTION OF COBRA VENOM DECREASES ADENOSINE DEAMINASE, HISTAMINE, AND IgE IN ORGANS OF MICE

Palavras-chave: Honeybee venom; Adenosine deaminase; Collagen; Histamine; IgE; Myoglobin; Nerve growth factor.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200006

The pharmacological substances adenosine deaminase (ADA), histamine, and IgE are endogenously present in animals. They are implicated in allergy and asthma and detectable in blood serum. This research reports the presence of ADA, histamine, and IgE at varying levels in almost all major organs of mice. This research further reports that intramuscular injection of sub-lethal dose of Naja kaouthia venom disrupted homeostasis and lowered the levels of ADA, histamine, and IgE in the organs of mice. Adult Balb/C male mice were injected with a half lethal dose of the venom. The mice were sacrificed at 2, 8, and 24 hours post-injection and different organs were collected. Organs were homogenized, centrifuged, and the supernatants were assayed for ADA, histamine,...

The presence of pharmacological substances myoglobin and histamine in venoms

Palavras-chave: Cobra venom; Balb/C mice organs; Adenosine deaminase; Histamine; IgE; Naja kaouthia.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000100006

LIPPS,B. V.; KHAN,A. A..

It is well documented that several pharmacological substances are released within the victim's body after snakebite. These substances are also believed to be endogenously present in animals, specifically levels of myoglobin and histamine that are reported to rise after envenomation. However, there is no published data regarding the presence of these substances in venoms per se. This research reports the detection of myoglobin and histamine in snake, scorpion, honeybee, and toad venoms by immunological test. It is unlikely that the rise in levels of myoglobin and histamine is due to that added from the bite, since a single toxin devoid of such components is capable of elevating levels of these substances. Nonetheless, it is likely that the rise in levels of...

In vitro assay of biological and chemical toxins using antibodies against lethal toxin neutralizing factor

Palavras-chave: Myoglobin; Histamine; Snake; Honeybee; Scorpion; Toad; Venom; Crotalus atrox; Crotalus polystictus; Naja kaouthia; Oxyuranus s. scutellatus; Actinopyga agassizi; Bufo arenarum; Androcetonus australis; Apis mellifera.

Ano: 2001

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000100004

Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently,...

Small synthetic peptides inhibit, in mice, the lethalithy of toxins derived from animal, plant and bacteria

Palavras-chave: Antibodies; In vitro assay; Lethal toxin neutralizing factor; Toxins; Venoms.

Ano: 2002

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200003

Lethal Toxin Neutralizing Factor (LTNF), isolated from opossum with having molecular weight 63 kDa, is a potent antidote for animal, plant, and bacterial toxins. This communication deals with the identification of a small fragment of LTNF eliciting the anti-lethal activity of animal, plant, and bacterial toxins when tested in mice. Purified LTNF was treated with trypsin to cause fragmentation at the arginine and lysine sites. The fragments were separated by high-performance liquid chromatography (HPLC) and were tested against anti-LTNF for binding affinity by enzyme-linked immunosorbent test (ELISA). The fragment showing the most binding to anti-LTNF was sequenced. Synthetic peptides consisting of 15 and 10 amino acids from the N-terminal were constructed...