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| Suquet, Marc; Agogue, Julie; Brizard, Raphael; Haffray, Pierrick; Labbe, Catherine; Le Coz, Jean-rene; Pepin, Jean-francois; Rault, Paul; Saulnier, Denis. |
| Ce rapport fait état d'une étude réalisée au laboratoire de Génétique et Pathologie de l'IFREMER dans le cadre de l'analyse d'épisodes de mortalité anormale survenue en écloserie privée de crevettes pénéides. Plusieurs hypothèses ont été avancées. La présence de Vibrio harveyi a amené à constituer une collection de 40 souches à partir desquelles une identification bactériologique a été effectuée. Elle montre en effet une dominance de souches appartenant à un groupe homogène de Vibrio harveyi. La technique de ribotypage (digestion d'ADN génomique, transfert de Southem-blot et hybridation avec une sonde spécifique) est alors mise en place. Plusieurs difficultés techniques ont ralenti l'analyse et n'ont donc pas permis d'avoir des résultats assez avancés pour... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Tétraploïdes; Sperme; Conservation; Diagnostic zoo-sanitaire; Flore bactérienne; Herpès virus.. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/00304/41554/40762.pdf |
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| Labbe, Catherine; Haffray, Pierrick; Mingant, Christian; Quittet, Benjamin; Diss, Blandine; Tervit, H. Robin; Adams, Serean L.; Rimond, Flore; Suquet, Marc. |
| Pacific oyster Crassostrea gigas is one major species for aquaculture, and the development of breeding programs and the need for preservation of wild stock genetic resources prompted the need for larvae cryopreservation. The objective of the present study was to choose the most reliable protocol from several existing publications, to test its biological and practical limitations, and to adapt it to hatchery conditions. The selected protocol was characterized by a very slow freezing rate without seeding, and by the use of ethylene glycol and sucrose as cryoprotectant. The best survivals after thawing and rearing up to 48 h post fertilization (hpf) were obtained with larvae that were frozen at late trochophore (20 hpf) and early-D (24 hpf) stages. Increasing... |
| Tipo: Text |
Palavras-chave: Oyster; Cryobanking; D stage; Ethylene glycol; High throughput; Hatchery. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00423/53460/54354.pdf |
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| Labbe, Catherine; Suquet, Marc; Michel, Gildas; Duclos, Delphine; Lemarchand, Alain; Le Hellec, Catherine; Vandeputte, Marc; Quittet, Benjamin; Goardon, Lionel; Pincent, Cedric; Haffray, Pierrick. |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00099/20979/18602.pdf |
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| Vandeputte, Marc; Labbe, Catherine; Suquet, Marc; Fauvel, Christian; Goardon, Lionel; Duclos, Delphine; Danchin-burge, Coralie; Vergnet, Alain; Rault, Paul; Degremont, Lionel; Benabdelmouna, Abdellah; Brizard, Raphael; Haffray, Pierrick. |
| Tipo: Text |
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| Ano: 2012 |
URL: http://archimer.ifremer.fr/doc/00098/20975/18597.pdf |
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| Brizard, Raphael; Bernardi, Maud; Boudry, Pierre; Haffray, Pierrick; Labbe, Catherine; Maisse, Gérard; Maurouard, Elise; Robert, Rene; Roger, Jean-luc. |
| Afin d'appuyer la filière ostréicole, les programmes de recherche actuels visent à proposer des méthodes d'amélioration génétique des rendements et de la qualité du produit (sélection, polyploïdisation). A l'image des productions animales terrestres, la continuation de ces travaux et la diffusion du progrès génétique dans la filière seraient grandement facilitée par le développement de techniques de conservation des génotypes sélectionnés. La cryopréservation qui est utilisée fréquemment dans les programmes de sélection des animaux terrestres et des espèces piscicoles n'est toujours pas disponible pour les mollusques bivalves. Cette technique présente les atouts qui peuvent pourraient satisfaire les différents acteurs de la filière ostréicole car elle... |
| Tipo: Text |
Palavras-chave: Congélation sperme; Standardisation; Procédure; Ecloserie expérimentale; Fécondation; Cryopréservation; Sperme cryopréservé. |
| Ano: 2004 |
URL: http://archimer.ifremer.fr/doc/00049/16056/13516.pdf |
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| Suquet, Marc; Labbe, Catherine; Puyo, Sophie; Mingant, Christian; Quittet, Benjamin; Boulais, Myrina; Queau, Isabelle; Ratiskol, Dominique; Diss, Blandine; Haffray, Pierrick. |
| This study is the first demonstration of successful post-thawing development to reproduction stage of diploid cryopreserved larvae in an aquatic invertebrate. Survival, growth and reproductive performances were studied in juvenile and adult Pacific oysters grown from cryopreserved embryos. Cryopreservation was performed at three early stages: trochophore (13 +/- 2 hours post fertilization: hpf), early D-larvae (24 +/- 2 hpf) and late D-larvae (43 +/- 2 hpf). From the beginning (88 days) at the end of the ongrowing phase (195 days), no mortality was recorded and mean body weights did not differ between the thawed oysters and the control. At the end of the growing-out phase (982 days), survival of the oysters cryopreserved at 13 +/- 2 hpf and at 43 +/- 2 hpf... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00190/30092/28635.pdf |
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| Martinez-paramo, Sonia; Horvath, Akos; Labbe, Catherine; Zhang, Tiantian; Robles, Vanesa; Herraez, Paz; Suquet, Marc; Adams, Serean; Viveiros, Ana; Tiersch, Terrence R.; Cabrita, Elsa. |
| This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of... |
| Tipo: Text |
Palavras-chave: Cryobanking; Aquatic animals; Fish gametes; Germplasm. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00341/45172/44567.pdf |
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