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Elimination of coliforms and Salmonella spp. in sheep meat by gamma irradiation treatment BJM
Henriques,Luciana Salles Vasconcelos; Henry,Fábio da Costa; Barbosa,João Batista; Ladeira,Silvania Alves; Pereira,Silvia Menezes de Faria; Antonio,Isabela Maria da Silva; Teixeira,Gina Nunes; Martins,Meire Lelis Leal; Vital,Helio de Carvalho; Rodrigues,Dália dos Prazeres; Reis,Eliane Moura Falavina dos.
This study aimed at evaluating the bacteriological effects of the treatment of sheep meat contaminated with total coliforms, coliforms at 45 °C and Salmonella spp. by using irradiation at doses of 3 kGy and 5 kGy. Thirty sheep meat samples were collected from animals located in Rio de Janeiro State, Brazil, and then grouped in three lots including 10 samples: non-irradiated (control); irradiated with 3 kGy; and irradiated with 5 kGy. Exposure to gamma radiation in a 137Cs source-driven irradiating facility was perfomed at the Nuclear Defense Section of the Brazilian Army Technological Center (CTEx) in Rio de Janeiro. The samples were kept under freezing temperature (-18 °C) until the analyses, which occurred in two and four months after irradiation. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food irradiation; Microbiology; Sheep meat quality.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400017
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Production and partial characterization of alkaline polygalacturonase secreted by thermophilic Bacillus sp. SMIA-2 under submerged culture using pectin and corn steep liquor Ciênc. Tecnol. Aliment.
Andrade,Marcela Vicente Vieira de; Delatorre,Andréia Boechat; Ladeira,Silvania Alves; Martins,Meire Lelis Leal.
Polygalacturonase production by the thermophilic Bacillus sp. SMIA-2 cultivated in liquid cultures containing 0.5% (w/v) apple pectin and supplemented with 0.3% (w/v) corn steep liquor, reached its maximum after 36 hours with levels of 39 U.mL-1. The increase in apple pectin and corn steep liquor concentrations in the medium from 0.5 and 0.3%, respectively, to 0.65%, markedly affected the production of polygalacturonase, whose activity increased four times, reaching a maximum of 150.3 U.mL-1. Studies on polygalacturonase characterization revealed that the optimum temperature of this enzyme was between 60-70 °C. Thermostability profile indicated that the enzyme retained about 82 and 63% of its activity at 60 and 70 °C, respectively, after 2 hours of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polygalacturonase; Corn steep liquor; Bacillus sp.; Thermophilic bacterium.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612011000100031
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