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	Provedor de dados BJMBR (2) Autor Leão,S.C. (2) Adan,C.B.D. (1) Carneiro,C.R.W. (1) Freitas,D. (1) Matsui,T. (1) Oliveira,R.S. (1) Sato,E.H. (1) Sousa,L.B. (1) Mais... Palavra-chave Animal model (1) Gene expression (1) Infectious eye diseases (1) Keratitis (1) Lamellar keratotomy (1) Mycobacterium chelonae (1) Mycobacterium tuberculosis (1) Phospholipase C (1) Promoters (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2004 (1) 2000 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity BJMBR Matsui,T.; Carneiro,C.R.W.; Leão,S.C.. The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstrated. The objective of the present study was to demonstrate that native PLC-a is expressed in M. tuberculosis H37Rv. Sera from mice immunized with recombinant PLC-a expressed in E. coli were used in immunoblots to evaluate PLC-a expression. The immune serum recognized a 49-kDa protein in immunoblots against M. tuberculosis extracts. No bands were visible in M. tuberculosis culture supernatants or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis; Phospholipase C; Gene expression; Promoters. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000001100003 An experimental model of mycobacterial infection under corneal flaps BJMBR Adan,C.B.D.; Sato,E.H.; Sousa,L.B.; Oliveira,R.S.; Leão,S.C.; Freitas,D.. In order to develop a new experimental animal model of infection with Mycobacterium chelonae in keratomileusis, we conducted a double-blind prospective study on 24 adult male New Zealand rabbits. One eye of each rabbit was submitted to automatic lamellar keratotomy with the automatic corneal shaper under general anesthesia. Eyes were immunosuppressed by a single local injection of methyl prednisolone. Twelve animals were inoculated into the keratomileusis interface with 1 µl of 10(6) heat-inactivated bacteria (heat-inactivated inoculum controls) and 12 with 1 µl of 10(6) live bacteria. Trimethoprim drops (0.1%, w/v) were used as prophylaxis for the surgical procedure every 4 h (50 µl, qid). Animals were examined by 2 observers under a slit lamp on the 1st,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious eye diseases; Keratitis; Animal model; Mycobacterium chelonae; Lamellar keratotomy. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000700010 Registros recuperados: 2 Primeira ... 1 ... Última

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