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Detection of Hepatitis E Virus in Sewage After an Outbreak on a French Island ArchiMer
Miura, Takayuki; Lhomme, Sebastien; Le Saux, Jean-claude; Le Mehaute, Philippe; Guillois, Yvonnick; Couturier, Elizabeth; Izopet, Jacques; Abranavel, Florence; Le Guyader, Soizick.
A hepatitis E outbreak, which occurred on a small isolated island, provided an opportunity to evaluate the association between the number of hepatitis E cases in the community and the concentration of virus detected in sewage. Samples were collected from the different sewage treatment plants from the island and analyzed for the presence of hepatitis E (HEV) virus using real-time RT-PCR. We demonstrated that if 1–4 % of inhabitants connected to a WWTP were infected with HEV, raw sewage contained HEV at detectable levels. The finding that such a small number of infected people can contaminate municipal sewage works raises the potential of the further distribution of the virus. Indeed, investigating the routes of transmission of HEV, including the potential...
Tipo: Text Palavras-chave: Hepatitis E virus; Wastewater treatment plant; Quantification.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00334/44482/44199.pdf
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Virus Type-Specific Removal in a Full-Scale Membrane Bioreactor Treatment Process ArchiMer
Miura, Takayuki; Schaeffer, Julien; Le Saux, Jean-claude; Le Mehaute, Philippe; Le Guyader, Soizick.
We investigated removal of noroviruses, sapoviruses, and rotaviruses in a full-scale membrane bioreactor (MBR) plant by monitoring virus concentrations in wastewater samples during two gastroenteritis seasons and evaluating the adsorption of viruses to mixed liquor suspended solids (MLSS). Sapoviruses and rotaviruses were detected in 25% of MBR effluent samples with log reduction values of 3- and 2-logs in geometric mean concentrations, respectively, while noroviruses were detected in only 6% of the samples. We found that norovirus and sapovirus concentrations in the solid phase of mixed liquor samples were significantly higher than in the liquid phase (P < 0.01, t test), while the concentration of rotaviruses was similar in both phases. The efficiency...
Tipo: Text Palavras-chave: Norovirus; Sapovirus; Rotavirus; MBR; Mixed liquor suspended solids; Adsorption.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00414/52548/53441.pdf
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