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| Azevedo,Fernando Alves de; Mourão Filho,Francisco de Assis Alves; Schinor,Evandro Henrique; Paoli,Luis Gustavo de; Mendes,Beatriz Madalena Januzzi; Harakava,Ricardo; Gabriel,Dean W.; Lee,Richard F.. |
| The objective of this work was the transformation of tobacco and 'Valencia' sweet orange with the GUS gene driven by the citrus phenylalanine ammonia-lyase (PAL) gene promoter (CsPP). Transformation was accomplished by co-cultivation of tobacco and 'Valência' sweet orange explants with Agrobacterium tumefaciens containing the binary vector CsPP-GUS/2201. After plant transformation and regeneration, histochemical analyses using GUS staining revealed that CsPP promoter preferentially, but not exclusively, conferred gene expression in xylem tissues of tobacco. Weaker GUS staining was also detected throughout the petiole region in tobacco and citrus CsPP transgenic plants. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xylella fastidiosa; PAL; Citrus variegated chlorosis; Cultivar resistance. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001100008 |
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| Iracheta-Cárdenas,María M.; Almeyda-León,Isidro H.; Cevik,Bayarm; Niblett,Charles L.; Lee,Richard F.; Rocha-Peña,Mario A.. |
| Citrus tristeza virus (CTV) is an important issue to the Mexican citrus industry. CTV infected plants have been reported in 20 citrus producing states of the country as symptomless virus infections, even on plants grafted on the sensitive sour orange rootstock. The detection of severe CTV isolates is mandatory to reinforce the operation of the CTV campaign nationwide. In this study, 13 CTV isolates from Nuevo León and one from Tamaulipas states were characterized for activity against the strain discriminatory MCA13 monoclonal antibody, bi-directional (BD) PCR, and hybridization with specific DNA probes, with RT-PCR amplicons, of the p25 coat protein gene. Ten out of 13 CTV isolates from Nuevo León and the Tamaulipas isolate gave a positive reaction to the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bi-directional PCR; DIBA; Hybridization; MCA 13 monoclonal antibody. |
| Ano: 2010 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000400005 |
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