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Micronuclei formation in liver fibrosis samples from patients infected by hepatitis C virus Genet. Mol. Biol.
Almeida,Terezinha M.B. de; Leitão,Regina Maria C.; Carrilho,Flair J.; Sonohara,Shigueko.
Genetic research on fibrosis outset and its progression in chronic hepatitis (CH) by hepatitis C virus (HCV) are limited. The lack of cytogenetic data led us to investigate the presence of micronuclei (MNi), as a sign of genomic damage. Hepatocytes of hepatic parenchyma from 62 cases diagnosed with CH associated with HCV and displaying different degrees of fibrosis (F1-F4) were analyzed. These data were compared to 15 cases without fibrosis (F0). Twelve healthy liver parenchyma samples were included as control. All samples were obtained from paraffin-embedded archival material. Micronucleated hepatocytes (MN-Heps) were analyzed through Feulgen/Fastgreen staining. Results showed that the rates of MN-Heps in the F4 group were statistically significant (p...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micronuclei; Hepatocytes; Chronic hepatitis C; Fibrosis progression; HCV-patients.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000300003
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