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| Mariano-da-Silva,Samuel; Brait,Joys Dias de Assis; Angeloni,Luiz Henrique Poleto; Mariano-da-Silva,Fabiana Maria de Siqueira; Leite,César Augusto Oliveira; Braga,Paula. |
| Sugar cane juice containing 12% (w.w -1) of total reducing sugars and 0.0 to 5.0 mmol of nickel L-1, with pH ranging from 3.5 to 6.5, was inoculated with Fleischmanns yeast (Saccharomyces cerevisiae) (10% w.w -1). Six hours after fermentation, the yeasts cellular viability and trehalose content were evaluated. The resulting must was centrifuged and the raw yeast was analyzed by atomic absorption spectroscopy to evaluate the intracellular levels of calcium, copper, iron, magnesium, manganese, nickel, phosphorus, potassium, sulfur and zinc. The intracellular levels of iron, magnesium and calcium were affected and the yeasts susceptibility to nickel was enhanced by the decrease in pH. The yeasts growth was not affected by nickel at high pH, but the toxic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nickel; Mineral composition; Saccharomyces cerevisiae; Yeast. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612007000300013 |
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| Mariano-da-Silva,Samuel; Oliveira,Silvio Luiz de; Leite,César Augusto Oliveira; Prado,Renata Silva do; Faria,Francys Pimenta de; Oliveira,Rangel Camillo Nunes; Mariano-da-Silva,Fabiana Maria de Siqueira. |
| The aim of this study was to evaluate the effects of pH, dextrose and yeast extract on the cadmium toxicity on Saccharomyces cerevisiae PE-2. In the first assay, the YED mediums with different pH (2, 3, 4, 5, 6, 7, and 8) containing 0.0 and 0.05 mmol Cd L-1 were inoculated with yeast suspension and incubated at 30 °C for 18 hours. During the anaerobic growth, the biomass concentration was determined. The yeast trehalose content, cell viability, and the growth rate were assessed at the beginning and at the end of the growth stages. In the second assay the YED mediums were diluted to the total, ½, and ¼ content of dextrose and yeast and 0.0 and 0.05 mmol Cd L-1 were added. The pH of the mediums was adjusted to 5. The culture mediums were inoculated and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Yeast; Cadmium; Dextrose; Yeast extract; PH. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612009000200009 |
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