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	Provedor de dados BJID (2) BJM (1) Autor Leite,Domingos da Silva (3) Blanco,Jesús (1) Carazzolle,Marcelo Falsarella (1) Moura,Claúdia de (1) Salvadori,Márcia Regina (1) Silveira,Wanderley Dias da (1) Stehling,Eliana Guedes (1) Tiba,Monique Ribeiro (1) Valadares,Geórgio Freesz (1) Yano,Tomomasa (1) Mais... Palavra-chave Antigens (1) Bacterial (1) Biological characterization (1) Calves (1) Cystic fibrosis (1) Diarrhea (1) E. coli (1) Escherichia coli (1) Extra-pulmonary infections (1) Pathogenesis (1) Polymerase chain reaction (1) Polymorphism (1) Pseudomonas aeruginosa (1) Restriction fragment length (1) Virulence factors (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2011 (1) 2008 (1) 2003 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Identification of putative new Escherichia coli flagellar antigens from human origin using serology, PCR-RFlP and DNA sequencing methods BJID Tiba,Monique Ribeiro; Moura,Claúdia de; Carazzolle,Marcelo Falsarella; Leite,Domingos da Silva. Escherichia coli has been isolated frequently, showing flagellar antigens that are not recognized by any of the 53 antisera, provided by the most important reference center of E. coli, The International Escherichia and Klebsiella Center (WHO) of the Statens Serum Institute, Copenhagen, Denmark. The objective of this study was to characterize flagellar antigens of E. coli that express non-typeable H antigens. The methods used were serology, PCR-RFLP and DNA sequencing. This characterization was performed by gene amplification of the fliC (flagellin protein) by polymerase chain reaction in all 53 standards E.coli strains for the H antigens and 20 E. coli strains for which the H antigen was untypeable. The amplicons were digested by restriction enzymes, and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Escherichia coli; Antigens; Bacterial; Polymerase chain reaction; Polymorphism; Restriction fragment length. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000200009 Study of biological characteristics of Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis and from patients with extra-pulmonary infections BJID Stehling,Eliana Guedes; Silveira,Wanderley Dias da; Leite,Domingos da Silva. A total of 120 strains of Pseudomonas aeruginosa, isolated from cystic fibrosis (CF) patients (n = 80) and from patients having extra-pulmonary infections (n = 40) were studied regarding the presence of some virulence factors (hemolysin, gelatinase and elastase production) and presence of the algD and algU genes as detected by polymerase chain reaction-PCR. There was not a significant difference for the production of gelatinase and hemolysin between non-mucoid strains from CF patients and other isolates from extra-pulmonary infections and mucoid strains. The production of elastase was found to be significant among these strains. The algD gene was detected by PCR in all studied strains but the algU gene was detected only in 25% of the mucoid strains.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biological characterization; Pathogenesis; Cystic fibrosis; Extra-pulmonary infections. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000100018 Virulence factors of Escherichia coli isolated from calves with diarrhea in Brazil BJM Salvadori,Márcia Regina; Valadares,Geórgio Freesz; Leite,Domingos da Silva; Blanco,Jesús; Yano,Tomomasa. Two hundred and five Escherichia coli strains isolated from calves with diarrhea from mid-western Brazil were screened for the presence of virulence factors associated with bovine colibacillosis. One hundred and two (49.8%) of the E. coli strains produced toxins: Shiga toxins 1 (9.7%) and 2 (6.3%), a-hemolysin (9.7%), enterohemolysin(6.8%), Cytotoxic Necrotizing Factors type 1 (0.5%), and type 2 (4.4%), enterotoxins LT-II (8.3%) and STa (3.9%). No strain produced enterotoxin LT-I. Fimbrial adhesins F5 and F17 were produced by 7.3% and 4.8% of the strains, respectivly, and none expressed F41. Seven strains (3.4%) possessed the gene eae and belonged to serotypes O26:H-; O111:H- and O118:H16. These results suggest that calves in Brazil may be an important... Tipo: Info:eu-repo/semantics/article Palavras-chave: Calves; Diarrhea; E. coli; Virulence factors. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300009 Registros recuperados: 3 Primeira ... 1 ... Última

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