|
|
|
|
|
| Lema, Anais Kimberley; Latimier, Marie; Nezan, Elisabeth; Fauchot, Juliette; Le Gac, Mickael. |
| The factors responsible for inducing the synthesis of toxins and responses from toxic phytoplankton blooms remain unclear. In this study we compare the influence of genotypic (at both the intra and interspecific levels) and environmental factors (nutrient concentration and ratio) on growth (in terms of cell densities) and domoic acid (DA) production in three Pseudo-nitzschia species: P. australis, P.pungens and P.fradulenta. A strong phosphate effect was detected. More precisely, a low initial concentration in phosphate, even at high initial nitrogen and silicate concentrations, induced the highest DA concentrations and the lowest cell densities in all strains/species studied. In contrast, a low initial concentration of nitrogen and silicate combined, with... |
| Tipo: Text |
Palavras-chave: Pseudo-nitzschia strains; Domoic acid; Ratio; Phosphate; Nitrogen. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00377/48849/49397.pdf |
| |
|
|
| Lema, Anais Kimberley; Metegnier, Gabriel; Quéré, Julien; Latimier, Marie; Youenou, Agnes; Lambert, Christophe; Fauchot, Juliette; Le Gac, Mickael. |
| Untangling the functional basis of divergence between closely related species is a step towards understanding species dynamics within communities at both the evolutionary and ecological scales. We investigated cellular (i.e: growth, domoic acid production, nutrient consumption) and molecular (transcriptomic analyses) responses to varying nutrient concentrations across several strains belonging to three species of the toxic diatom genus Pseudo-nitzschia. Three main results were obtained. First, strains from the same species displayed similar transcriptomic, but not necessarily cellular, responses to the experimental conditions. It showed the importance of considering intra-specific diversity to investigate functional divergence between species. Second, a... |
| Tipo: Text |
Palavras-chave: Phenotype; Transcriptomics; Pseudo-nitzschia; Divergence; Nutrients. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00482/59392/62157.pdf |
| |
|
|
| Lema, Anais Kimberley; Clode, Peta L.; Kilburn, Matt R.; Thornton, Ruth; Willis, Bette L.; Bourne, David G.. |
| Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N-2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of N-15-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations.... |
| Tipo: Text |
|
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00664/77573/79543.pdf |
| |
|
|
|
