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	Provedor de dados ArchiMer (5) Autor Leroy, Celine (5) Compere, Chantal (4) Delbarre Ladrat, Christine (4) Combes, D (2) Combes, Didier (2) Ghillebaert, F (2) Ghillebaert, François (2) Raguenes, Gerard (1) Rochet, Marie-joelle (1) Mais... Palavra-chave Antifouling (3) Adhesion (2) DAPI (2) Marine biofilm (2) Microtiter plate (2) Pseudoalteromonas (2) Screening (2) Adhésion (1) Antisalissure (1) Biofilm (1) Biofilm marin (1) Capsular protein (1) Criblage (1) EPS (1) Exopolymer (1) Fouling (1) Hydrolases (1) Microplaque (1) Pseudoalteromonas sp. (1) Salissure marine (1) Mais... Tipo do documento Text (5) Ano 2008 (1) 2007 (1) 2006 (1) 2005 (2) País France (5) Idioma Inglês (3) Francês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última Lutte contre les salissures marines : approche par procédés enzymatiques. ArchiMer Leroy, Celine. Fouling on marine underwater surfaces causes critical and economic problems such as important material biodamages and industrial performances reduction. We chose to test antifouling potential of enzymatic commercial preparations like hydrolases (proteases, glycosidases and lipases) in order to inhibit the first fouling adhesion step: bacterial biofilm formation. An evaluation test of antifouling properties onto marine bacterial adhesion was designed using a mono-incubation of Pseudoalteromonas sp. D41 in microtiter plate and in sterile natural sea water. This test was adapted to screen agents for bacterial adhesion removal or inhibition activities and allowed to test enzymatic preparations toxicity on non adhered bacteria. Inhibition rates according to... Tipo: Text Palavras-chave: Fouling; Adhesion; Antifouling; Hydrolases; EPS; DAPI; Microtiter plate; Screening; Pseudoalteromonas sp.; Marine biofilm; Salissure marine; Adhésion; Antisalissure; Hydrolases; EPS; DAPI; Microplaque; Criblage; Pseudoalteromonas sp.; Biofilm marin. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/these-1099.pdf Adhésion et substances polymériques extracellulaires d’une nouvelle espèce bactérienne marine ArchiMer Leroy, Celine; Compere, Chantal; Delbarre Ladrat, Christine; Raguenes, Gerard; Ghillebaert, François; Combes, Didier. Une nouvelle bactérie marine du genre Pseudoalteromonas sp., D41, formant rapidement des biofilms sur tout type de matériau (verre, acier inoxydable, Téflon) a été isolée en Baie de Brest. L’objectif de ce présent travail est de caractériser et décrire cette bactérie capable de produire des exopolymères constitués en majorité de protéines. Le rôle des protéines sécrétées, dans les mécanismes d’adhésion, est discuté. Tipo: Text Ano: 2005 URL: http://archimer.ifremer.fr/doc/00088/19950/17617.pdf A biofilm-forming marine bacterium producing proteins as extracellular polymeric substances ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, François; Compere, Chantal; Combes, Didier. EPS biochemical characterization and observation by epifluorescencemicroscopy. Development of a marine biofilmformation model onto a polystyrene microtiterplate using the “O’Tooleand Koltermethod”[1] adapted with the well known fluorescent DNA staining DAPI Tipo: Text Ano: 2005 URL: http://archimer.ifremer.fr/doc/00089/19998/17661.pdf A marine bacterial adhesion microplate test using the DAPI fluorescent dye: a new method to screen antifouling agents ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Rochet, Marie-joelle; Compere, Chantal; Combes, D. Aims: To develop a method to screen antifouling agents against marine bacterial adhesion as a sensitive, rapid, quantitative microplate fluorescent test. Methods and Results: Our experimental method is based on a natural biofilm formed by mono-incubation of the marine bacterium Pseudoalteromonas sp. D41 in sterile natural sea water in a 96 wells polystyrene microplate. The 4'6-diamidino-2-phenylindole (DAPI) dye was used to quantify adhered bacteria in each well. Total measured fluorescence in wells was correlated with the amount of bacteria showing a detection limit of one bacterium per 5 µm2 and quantifying 2 x 107 to 2 x 108 bacteria adhered per cm2. The antifouling properties of three commercial surface active agents and chlorine were tested by this... Tipo: Text Palavras-chave: Antifouling; Pseudoalteromonas; Screening; Microtiter plate; Adhesion; DAPI; Marine biofilm. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2006/publication-2017.pdf Influence of subtilisin on the adhesion of a marine bacterium which produces mainly proteins as extracellular polymers ArchiMer Leroy, Celine; Delbarre Ladrat, Christine; Ghillebaert, F; Compere, Chantal; Combes, D. Aims: The nature of exopolymers involved in the adhesion of a marine biofilm-forming bacterium Pseudoalteromonas sp. D41 was investigated to evaluate and understand the antifouling potential of subtilisin. Methods and Results: The exopolymers of D41 produced by fermentation were analysed by FTIR and SDS-PAGE showing the presence of polysaccharides, glycoproteins and proteins. A high content of proteins was detected both in soluble and capsular fractions. The microscopic observations of fluorescamine and calcofluor stained adhered D41 indicated mainly the presence of proteins in exopolymers produced during adhesion. Subtilisin, the broad spectrum protease, tested in natural sea water and in polystyrene microplates showed that antifouling activity was... Tipo: Text Palavras-chave: Subtilisin; Pseudoalteromonas; Exopolymer; Capsular protein; Biofilm; Antifouling. Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4624.pdf Registros recuperados: 5 Primeira ... 1 ... Última

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