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	Provedor de dados Biol. Res. (2) BJM (1) Autor Li,Hui (3) Chen,Haide (1) Cui,Chun (1) Cui,Di (1) Han,Siqin (1) Li,JinLong (1) Li,Yang (1) Lin,Xijuan (1) Wang,NianYue (1) Xiao,Lei (1) Xu,Hui (1) Zhang,Ying (1) Zhang,Yong Chen (1) Zhang,ZhaoLi (1) Zhou,Lisha (1) Mais... Palavra-chave CIITA (1) Degradation (1) Genus (1) HESCs (1) Immune rejection (1) Interleukin IL-2 (1) Polycyclic aromatic hydrocarbons (PAHs) (1) Prostate cancer cells PC3 (1) Sphingomonas (1) TALENs (1) Trichosanthin (1) Tumor (1) Mais... Tipo do documento Journal article (2) Info:eu-repo/semantics/article (1) Ano 2016 (2) 2015 (1) País Chile (2) Brazil (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Functional disruption of human leukocyte antigen II in human embryonic stem cell Biol. Res. Chen,Haide; Li,Yang; Lin,Xijuan; Cui,Di; Cui,Chun; Li,Hui; Xiao,Lei. BACKGROUND: Theoretically human embryonic stem cells (hESCs) have the capacity to self-renew and differentiate into all human cell types. Therefore, the greatest promise of hESCs-based therapy is to replace the damaged tissues of patients suffering from traumatic or degenerative diseases by the exact same type of cells derived from hESCs. Allo-graft immune rejection is one of the obstacles for hESCs-based clinical applications. Human leukocyte antigen (HLA) II leads to CD4+ T cells-mediated allograft rejection. Hence, we focus on optimizing hESCs for clinic application through gene modification RESULTS: Transcription activator-like effector nucleases (TALENs) were used to target MHC class II transactivator (CIITA) in hESCs efficiently. CIITA-/-hESCs did... Tipo: Journal article Palavras-chave: HESCs; CIITA; TALENs; Immune rejection. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100059 The anti-cancerous activity of recombinant trichosanthin on prostate cancer cell PC3 Biol. Res. Li,JinLong; Li,Hui; Zhang,ZhaoLi; Wang,NianYue; Zhang,Yong Chen. CONTEXT: Trichosanthin produced in the root tube of Trichosanthes kirilowii shows anti-tumor activity on a series of cancer cells including Hela, MCF-7, HL-60. But there is little information about its effect on the carcinogenesis of prostate cancer. OBJECTIVE: This work was designed to study the role of trichosanthin on prostate cancer cells PC3. MATERIALS AND METHODS: Trichosanthin was expressed in BL21 strain and purified by affinity chromatography. MTT assay was designed to determine the effect of trichosanthin on growth of PC3 cells at doses of 10, 20, 40, 60, 80, and 120 &#956;g/ml.Then the effect of 50 &#956;g/ml rTCS alone or combined with 2 &#956;&#924; IL-2 on PC3 cell proliferation was analyzed. And the mechanism of rTCS was... Tipo: Journal article Palavras-chave: Trichosanthin; Interleukin IL-2; Prostate cancer cells PC3; Tumor. Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602016000100021 Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities BJM Zhou,Lisha; Li,Hui; Zhang,Ying; Han,Siqin; Xu,Hui. Abstract Members of the Sphingomonas genus are often isolated from petroleum-contaminated soils due to their unique abilities to degrade polycyclic aromatic hydrocarbons (PAHs), which are important for in situ bioremediation. In this study, a combined phenotypic and genotypic approach using streptomycin-containing medium and Sphingomonas -specific PCR was developed to isolate and identify culturable Sphingomonas strains present in petroleum-contaminated soils in the Shenfu wastewater irrigation zone. Of the 15 soil samples examined, 12 soils yielded yellow streptomycin-resistant colonies. The largest number of yellow colony-forming units (CFUs) could reach 105 CFUs g-1 soil. The number of yellow CFUs had a significant positive correlation (p < 0.05)... Tipo: Info:eu-repo/semantics/article Palavras-chave: Sphingomonas; Polycyclic aromatic hydrocarbons (PAHs); Degradation; Genus. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200271 Registros recuperados: 3 Primeira ... 1 ... Última

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