|
|
|
|
|
| Li,X; Liu,L; Li,Q; Xu,G; Zheng,J. |
| ABSTRACT Salmonella enterica subspecies enterica serotype Enteritidis (SE) has caused foodborne infections over decades. It is transmitted mainly from contaminated eggs to humans. SE is commonly present in layer houses, and closely interacts with environmental factors. The objective of the present study was to develop a viable PCR method to identify SE in environmental samples collected in layer farms of different sizes, and to evaluate SE contamination status in four main egg-production provinces of northern China. After specificity retrieval using Primer-BLAST against the NCBI database, three SE specific oligonucleotide primers were selected as candidate primers. The primers targeting Prot6e gene were adopted and primers targeting Sdf I were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Environment; Layer farm; PCR; Salmonella Enteritidis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300377 |
| |
|
|
| Shen,X; Wang,Y; Cui,C; Zhao,X; Li,D; Zhu,Q; Jiang,X; Yang,C; Qiu,M; Yu,C; Li,Q; Du,H; Zhang,Z; Yin,H. |
| ABSTRACT The melanocortin 1 receptor (MC1R) gene plays a key role in controlling the deposition of melanin. In mammals, the MC1Rgene is regarded as a major candidate gene in the control of melanin formation. In domestic animals, the MC1R gene mainly controls the expression of coat, skin, and plumage color in mammals and birds. In order to breed chickens with dark-green shank faster, we screened the molecular markers for shank color in a HS chicken population by exploring the relationship between polymorphism of the MC1R gene and three different shank colors (light green, dark green and yellow). Two primer pairs for code region of the MC1R gene were designed in the basic of chicken genomic sequence. DNA sequencing was performed to detect the polymorphisms... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HS chicken; MC1R gene; Polymorphisms; Shank color.. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000300314 |
| |
|
|
| Gao,G; Wang,H; Zhao,X; Li,Q; Wang,C; Li,J; Zhang,K; Zhong,H; Wang,Q. |
| ABSTRACT The objective of this work was to investigate the effect of feeding conditions on methylation status of FATP1 gene, which is an important candidate gene of Intramuscular fat and important indicator of chicken meat quality. We selected Daninghe (DNH) and Qingjiaoma (QJM) chickens under scatter-feeding and captivity-feeding conditions as experimental animals, and detected the methylation status of FATP1 genes in chicken breast muscle using Bisulfite Sequencing PCR method. The results showed that the methylation level of FATP1 in scatter-fed chicken was lower than in captivity-fed conditions in DNH and QIM chicken breast tissues; DNA methylation in the promoter and exon1 region was demonstrated to negatively regulate the expression of the FATP1 gene.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Feeding conditions; Chicken; FATP1; Meat quality; Intramuscular fat. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000500055 |
| |
|
|
| Yu,C; Qiu,M; Jiang,X; Zhang,Z; Du,H; Li,Q; Xia,B; Song,X; Hu,C; Xiong,X; Yang,L; Peng,H; Chen,J; Wang,Y; Yang,C. |
| ABSTRACT Chinese indigenous chicken breeds are geographically widespread, and a total of 116 indigenous chicken breeds are listed as Chinese national genetic resources. However, these indigenous chicken breeds are facing serious challenges as declining population and germplasm degeneration because lots of commercial chicken breeds had been introduced. In this study, the genetic variations of eleven Chinese indigenous chicken breeds of Sichuan province and three commercial chicken breeds were investigated based on the partial mitochondrial DNA D-loop of 487bp in length. 147 individuals from 14 breeds were examined and 34 haplotypes were observed. Genetic diversity analysis showed that the highest haplotype diversity level was found in Dahen Chicken (DH)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chinese chicken; Mitochondrial DNA; Cluster analysis; Gene flow; Phylogeny. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400308 |
| |
|
|
|
