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	Provedor de dados BABT (1) Electron. J. Biotechnol. (1) Autor Li,Shanshan (2) Wang,Zhigang (2) Mo,Jixian (1) Tang,Xiaobo (1) Xu,Weihui (1) Yu,Lei (1) Yu,Zhidan (1) Zhang,Rong (1) Zhu,Daling (1) Mais... Palavra-chave 15-hydroxyeicosatetraenoic acid (1) Bacillus (1) Box-Behnken design (1) Dimethyl phthalate (1) Hypoxia (1) Medium optimization (1) Plackett-Burman design (1) Pulmonary artery rings (1) Pulmonary artery smooth muscle cells (1) [ Ca2+]i (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2015 (1) 2008 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Enhanced production of dimethyl phthalate-degrading strain Bacillus sp. QD14 by optimizing fermentation medium Electron. J. Biotechnol. Mo,Jixian; Wang,Zhigang; Xu,Weihui; Li,Shanshan; Yu,Zhidan. Background Integrated statistical experimental designs were applied to optimize the medium constituents for the production of a dimethyl phthalate (DMP)-degrading strain Bacillus sp. QD14 in shake-flask cultures. A Plackett-Burman design (PBD) was applied to screen for significant factors, followed by the Steepest Ascent Method (SAM) to find the nearest region of maximum response. A Box-Behnken design (BBD) of the Response Surface Methodology (RSM) was conducted to optimize the final levels of the medium components. Results After the regression equation and response surface contour plots were analyzed, the concentrations of glucose, corn meal and NaCl were found to significantly influence the biomass of DMP-degrading bacteria. A combination of 22.88 g/L of... Tipo: Journal article Palavras-chave: Bacillus; Box-Behnken design; Dimethyl phthalate; Medium optimization; Plackett-Burman design. Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300016 Influence of the 15-HETE on cytosolic [ Ca2+] i of the rabbit pulmonary artery smooth muscle cells BABT Tang,Xiaobo; Zhang,Rong; Wang,Zhigang; Yu,Lei; Li,Shanshan; Zhu,Daling. Extracellular Ca2+ influx was blocked by L-type Ca2+ channel blocker nifedipine to observe the effects of 15-hydroxyeicosatetraenoic acid on the constriction of rabbit pulmonary artery rings and on the changes of Ca2+ level in the rabbit pulmonary artery smooth muscle cells, and further to investigate the mechanism of the calcium mobilization induced by the 15-HETE under hypoxic conditions. The effect of extracellular Ca2+ on tension of the rabbit PA rings was also studied. Nifedipine (10 µ mol/L) had no effect on 1 µ mol/L 15-hydroxyeicosatetraenoic acid induced vasoconstriction under normoxic and hypoxic conditions. Intracellular Ca2+ increased markedly in the 15-HETE group (cells were exposed to 1 µ mol/L 15-HETE for 8 min during culture) compared to... Tipo: Info:eu-repo/semantics/article Palavras-chave: 15-hydroxyeicosatetraenoic acid; Hypoxia; Pulmonary artery rings; Pulmonary artery smooth muscle cells; [ Ca2+]i. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200007 Registros recuperados: 2 Primeira ... 1 ... Última

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