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Characterization of Klebsiella pneumoniae associated with cattle infections in southwest China using multi-locus sequence typing (MLST), antibiotic resistance and virulence-associated gene profile analysis BJM
Cheng,Fangjun; Li,Zhangcheng; Lan,Shimei; Liu,Wei; Li,Xiaoyan; Zhou,Zuoyong; Song,Zhenhui; Wu,Juan; Zhang,Manli; Shan,Wenjie.
Abstract Klebsiella pneumoniae is important human and animal pathogen that causes a wide spectrum of infections. In this study, isolates from cattle nasal swabs samples were identified by 16S rRNA, and to evaluate the antimicrobial susceptibility, virulence gene carrying levels, and multilocus sequence typing of K. pneumoniae isolates. 33 isolates of K. pneumoniae were isolated and identified in 213 nasal swabs samples, of which 12 were hypervirulent K. pneumoniae strains. Extended Spectrum Beta-Lactamases genes were found in 93.4% of the strains. Of which, TEM was the most prevalent (93.4%), followed by CTX-M and SHV were 57.6% and 39.4%, respectively. A main mutation pattern of quinoloneresistance-determining region, Thr83-Ieu and Asp87-Asn in gyrA and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cattle; Antimicrobial susceptibility; Klebsiella pneumonia; MLST; Virulence genes.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500093
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Effects of open drainage ditch design on bacterial and fungal communities of cold waterlogged paddy soils BJM
Qiu,Shanlian; Wang,MK; Wang,Fei; Chen,Jichen; Li,Xiaoyan; Li,Qinghua; Lin,Cheng; Lin,Xinjian.
A field experiment established in 1980 was conducted to evaluate the effects of open drainage ditch applied for water removal on bacterial and fungal communities of cold waterlogged paddy soils in 2011. In this experiment, traditional plate counting and temperature gradient gel electrophoresis were employed to characterize the abundance and diversity of soil bacterial and fungal communities. Four different distances from the open drainage ditch, 5, 15, 25 and 75 m with different degrees of drainage were designed for this study. Maximum populations of culturable aerobic bacteria and fungi were at 15-m distance while minimum populations were at 75-m distance. Significant differences (p < 0.05) in fungal populations were observed at all distances from open...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abundance; Bacterial and fungal communities; Diversity; Open drainage ditch.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300050
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