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| Ramírez-Carreto,Santos; Pérez-García,Erick I.; Salazar-García,Sandra I.; Bernáldez-Sarabia,Johanna; Licea-Navarro,Alexei; Rudiño-Piñera,Enrique; Pérez-Martínez,Leonor; Pedraza-Alva,Gustavo; Rodríguez-Almazán,Claudia. |
| Abstract Background: Pore-forming proteins (PFP) are a class of toxins abundant in the venom of sea anemones. Owing to their ability to recognize and permeabilize cell membranes, pore-forming proteins have medical potential in cancer therapy or as biosensors. In the present study, we showed the partial purification and sequencing of a pore-forming protein from Anthopleura dowii Verrill (1869). 17. Methods: Cytolytic activity of A. dowii Verrill (1869) venom was determined via hemolysis assay in the erythrocytes of four mammals (sheep, goat, human and rabbit). The cytotoxic activity was analyzed in the human adherent lung carcinoma epithelial cells (A549) by the cytosolic lactate dehydrogenase (LDH) assay, and trypan blue staining. The venom was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anthopleura; Pore-forming protein; Venom; Lung carcinoma; Sea anemone. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100301 |
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