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	Provedor de dados BABT (1) BJM (1) Autor Araújo,Elza Fernandes de (2) Lima,Juliana Oliveira (2) Queiroz,Marisa Vieira de (2) Lopes,Francis Julio Fagundes (1) Pereira,Jorge Fernando (1) Silva,Viviane Aline Oliveira (1) Mais... Palavra-chave 2-Deoxy-d-glucose (1) Moniliophthora perniciosa (1) Pectinolytic complex (1) Restriction enzyme (1) Spontaneous mutant (1) Transformation (1) Ultraviolet mutagenesis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2017 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Restriction enzyme improves the efficiency of genetic transformations in Moniliophthora perniciosa, the causal agent of witches’ broom disease in Theobroma cacao BABT Lopes,Francis Julio Fagundes; Queiroz,Marisa Vieira de; Lima,Juliana Oliveira; Silva,Viviane Aline Oliveira; Araújo,Elza Fernandes de. The presence of restriction enzymes in the transformation mixture improved the efficiency of transformation in Moniliophthora perniciosa. The influence of the vector shape (linear or circular), the patterns of plasmid integration in genomic sites and the influence of the promoter used to express the gene marker were also analyzed. The addition of BamHI or NotI increased the number of transformants by 3-10-fold and 3-fold, respectively, over the control without added enzyme. The use of pre-linearized plasmid did not increase the transformation efficiency in comparison with the circular plasmid. However, the frequency of multi-copy transformants increased significantly. The transformation procedure here reported resulted in better production of protoplasts... Tipo: Info:eu-repo/semantics/article Palavras-chave: Moniliophthora perniciosa; Transformation; Restriction enzyme. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000100004 Pectin lyase overproduction by Penicillium griseoroseum mutants resistant to catabolite repression BJM Lima,Juliana Oliveira; Pereira,Jorge Fernando; Araújo,Elza Fernandes de; Queiroz,Marisa Vieira de. Abstract Expression of pectinolytic genes is regulated by catabolic repression limiting the production of pectin lyase (PL) if the natural inducer, pectin, is missing from the growth medium. Here, we report the isolation of Penicillium griseoroseum mutants resistant to 2-deoxy-d-glucose (DG) that show resistance to catabolite repression and overproduce PL. Three spontaneous and nine UV-induced mutants were obtained. Some mutants produced sectors (segments morphologically different) that were also studied. The mutants were analyzed for pectinases production on pectinase-agar plates and five mutants and two sectors showing larger clearing zones than the wild type were selected for quantitative assay. Although PL production higher than the wild type has been... Tipo: Info:eu-repo/semantics/article Palavras-chave: 2-Deoxy-d-glucose; Ultraviolet mutagenesis; Pectinolytic complex; Spontaneous mutant. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300602 Registros recuperados: 2 Primeira ... 1 ... Última

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