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| Fuscaldi,Leonardo Lima; Santos,Daniel Moreira dos; Pinheiro,Natália Gabriela Silva; Araújo,Raquel Silva; Barros,André Luís Branco de; Resende,Jarbas Magalhães; Fernandes,Simone Odília Antunes; Lima,Maria Elena de; Cardoso,Valbert Nascimento. |
| Abstract Background Current diagnostic methods and imaging techniques are not able to differentiate septic and aseptic inflammation. Thus, reliable methods are sought to provide this distinction and scintigraphic imaging is an interesting option, since it is based on physiological changes. In this context, radiolabeled antimicrobial peptides have been investigated as they accumulate in infectious sites instead of aseptic inflammation. The peptide LyeTx I, from the venom of Lycosa erythrognatha, has potent antimicrobial activity. Therefore, this study aimed to synthesize LyeTx I derivatives with the chelating compound HYNIC, to evaluate their antimicrobial activity and to radiolabel them with 99mTc. Methods Two LyeTx I derivatives, HYNIC-LyeTx I... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Septic and aseptic inflammation; Differential diagnosis; Antimicrobial peptides LyeTx I derivatives; MALDI-ToF-MS RP-HPLC; Technetium-99m; HYNIC EDDA Tricine. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992016000100312 |
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| Silva,Flavia Rodrigues da; Paiva,Mayara Rodrigues Brandão de; Dourado,Lays Fernanda Nunes; Silva,Rummenigge Oliveira; Silva,Carolina Nunes da; Costa,Bruna Lopes da; Toledo,Cibele Rodrigues; Lima,Maria Elena de; Silva-Cunha,Armando da. |
| Abstract Background: The great diversity of molecules found in spider venoms include amino acids, polyamines, proteins and peptides, among others. Some of these compounds can interact with different neuronal receptors and ion channels including those present in the ocular system. To study potential toxicity and safety of intravitreal injection in rabbits of LyeTx I b, a synthetic peptide derived from the toxin LyeTx I found in venom from the spider Lycosa eritrognatha and to evaluate the angiogenic activity on a CAM model. Methods: ARPE-19 cells were treated with LyeTx I b (0.36; 0.54; 0.72; 2.89; 4.34 or 9.06 μM). In this study, New Zealand rabbits were used. LyeTx I b (2.89 μM) labeled with FITC dissolved in PBS, or only PBS, were injected into... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lycosa eritrognatha; LyeTx I b; Intravitreal injection; Retinal diseases; Toxicity; Retinal neovascularization. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100322 |
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| Gusmão,Karla A. G.; Santos,Daniel M. dos; Santos,Virgílio M.; Cortés,María Esperanza; Reis,Pablo V. M.; Santos,Vera L.; Piló-Veloso,Dorila; Verly,Rodrigo M.; Lima,Maria Elena de; Resende,Jarbas M.. |
| Abstract Background The availability of antimicrobial peptides from several different natural sources has opened an avenue for the discovery of new biologically active molecules. To the best of our knowledge, only two peptides isolated from the frog Leptodactylus labyrinthicus, namely pentadactylin and ocellatin-F1, have shown antimicrobial activities. Therefore, in order to explore the antimicrobial potential of this species, we have investigated the biological activities and membrane interactions of three peptides isolated from the anuran skin secretion. Methods Three peptide primary structures were determined by automated Edman degradation. These sequences were prepared by solid-phase synthesis and submitted to activity assays against gram-positive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptodactylus labyrinthicus; Ocellatins; Antimicrobial peptides; Peptide membrane interactions. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100302 |
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| Oliveira,Camila Franco Batista; Alves,Daniela Pereira; Emerich,Bruna Luiza; Figueiredo,Suely Gomes de; Cordeiro,Marta do Nascimento; Borges,Márcia Helena; Richardson,Michael; Pimenta,Adriano Monteiro de Castro; Duarte,Igor Dimitri Gama; Lima,Maria Elena de. |
| ABSTRACT Background: The venom of Phoneutria nigriventer spider is a source of numerous bioactive substances, including some toxins active in insects. An example is PnTx4(5-5) that shows a high insecticidal activity and no apparent toxicity to mice, although it inhibited NMDA-evoked currents in rat hippocampal neurons. In this work the analgesic activity of PnTx4(5-5) (renamed Γ-ctenitoxin-Pn1a) was investigated. Methods: The antinociceptive activity was evaluated using the paw pressure test in rats, after hyperalgesia induction with intraplantar injection of carrageenan or prostaglandin E2 (PGE2). Results: PnTx4(5-5), subcutaneously injected, was able to reduce the hyperalgesia induced by PGE2 in rat paw, demonstrating a systemic effect. PnTx4(5-5)... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Spider toxin; Γ-ctenitoxin-Pn1a; PnTx4(5-5); Phoneutria nigriventer; Antinociception; Glutamate. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100316 |
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