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	Provedor de dados BJMBR (2) Autor Lin,Y. (2) Chen,D.P. (1) Cui,Y. (1) Diao,Y.P. (1) Liu,L. (1) Peng,J. (1) Peng,M. (1) Yao,Q.Y. (1) Ye,D.M. (1) Zhou,P. (1) Zhou,Y. (1) Mais... Palavra-chave Bidirectional modulation (1) Bioinformatics (1) Calcium dependent (1) Cloning (1) Der f 1 (1) Dermatophagoides farinae (1) Enteric nervous system (1) Expression (1) Homeostasis (1) Intestinal motility (1) Recombinant allergens (1) Taurine (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Cloning, sequence analysis, and expression of cDNA coding for the major house dust mite allergen, Der f 1, in Escherichia coli BJMBR Cui,Y.; Zhou,P.; Peng,J.; Peng,M.; Zhou,Y.; Lin,Y.; Liu,L.. Our objective was to clone, express and characterize adult Dermatophagoides farinae group 1 (Der f 1) allergens to further produce recombinant allergens for future clinical applications in order to eliminate side reactions from crude extracts of mites. Based on GenBank data, we designed primers and amplified the cDNA fragment coding for Der f 1 by nested-PCR. After purification and recovery, the cDNA fragment was cloned into the pMD19-T vector. The fragment was then sequenced, subcloned into the plasmid pET28a(+), expressed in Escherichia coli BL21 and identified by Western blotting. The cDNA coding for Der f 1 was cloned, sequenced and expressed successfully. Sequence analysis showed the presence of an open reading frame containing 966 bp that encodes a... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dermatophagoides farinae; Der f 1; Recombinant allergens; Cloning; Expression; Bioinformatics. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008000500006 Modulatory effects of taurine on jejunal contractility BJMBR Yao,Q.Y.; Chen,D.P.; Ye,D.M.; Diao,Y.P.; Lin,Y.. Taurine (2-aminoethanesulfonic acid) is widely distributed in animal tissues and has diverse pharmacological effects. However, the role of taurine in modulating smooth muscle contractility is still controversial. We propose that taurine (5-80 mM) can exert bidirectional modulation on the contractility of isolated rat jejunal segments. Different low and high contractile states were induced in isolated jejunal segments of rats to observe the effects of taurine and the associated mechanisms. Taurine induced stimulatory effects on the contractility of isolated rat jejunal segments at 3 different low contractile states, and inhibitory effects at 3 different high contractile states. Bidirectional modulation was not observed in the presence of verapamil or... Tipo: Info:eu-repo/semantics/article Palavras-chave: Taurine; Bidirectional modulation; Enteric nervous system; Calcium dependent; Homeostasis; Intestinal motility. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001201068 Registros recuperados: 2 Primeira ... 1 ... Última

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