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| Ling,Zheng; Qi-Qing,Jiao; Yu,Wang; Fei,Bian; Shu-Jie,Qu; Shu-Bo,Wan; Zhen-Ying,Peng; Yu-Ping,Bi. |
| The increased incidence of diabetes, coupled with the introduction of alternative insulin delivery methods that rely on higher doses, is expected to result in a substantial escalation in the future demand for affordable insulin. Plant-based systems offer a safe and economical method for producing pharmaceutical proteins. We used peanut (Arachis hypogaea L.) as bio-reactors to express biosafe, stable proinsulin. We designed two proinsulin analogues (FAIA and LAIA) with substitutions in their amino acid sequences. The fast-acting insulin analogue (FAIA) contains a Gly inserted between Cys19 and Gly20, as well as a Pro28Asp substitution, in the B chain. The long-acting insulin analogue (LAIA) contains a Gly inserted between Cys19 and Gly20 and two Arg... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Proinsulin; Peanut; Diabetes; Expression. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100323 |
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