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| Wu,Xiang-mei; Liu,Xing; Bu,You-quan; Sengupta,Joyeeta; Cui,Hong-juan; Yi,Fa-ping; Liu,Tao; Yuan,Chen-fu; Shi,Yan-yan; Song,Fang-zhou. |
| The oncogene Bmi-1 is a member of the Polycomb group gene family. Its expression is found to be greatly increased in a number of malignant tumors including breast cancer. This could suggest Bmi-1 as a potent therapeutic target. In this study, RNAi was introduced to down-regulate the expression of Bmi-1 in a highly malignant breast adenocarcinoma cell line, MCF-7. A thorough study of the biological behavior and chemosensitivity changes of the MCF-7 cells was carried out in context to the therapeutic potential of Bmi-1. The results obtained indicated that siRNA targeting of Bmi-1 could lead to an efficient and specific inhibition of endogenous Bmi-1 activity. The mRNA and protein expression of Bmi-1 were determined by RT-PCR and Western blot, respectively.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: RNA interference; Bmi-1; Retrovirus vector; Doxorubicin; Breast cancer. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400004 |
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