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Artigas, Luis Felipe; Didry, Morgane; Lampert, Luis; Broutin, Mathias; Bonato, Simon; Lizon, Fabrice; Lefebvre, Alain. |
L'élément de qualité phytoplancton doit être évalué dans le cadre de la Directive Cadre sur L’Eau grâce à trois indices : biomasse, abondance et composition. Les deux premiers indices, biomasse et abondance, ont déjà été définis. L’indice composition a quant à lui fait l'objet d'une proposition précise en Méditerranée, mais pas en Manche - Atlantique. Les travaux antérieurs montrent que la construction d'un indice de composition doit tenir compte de la diversité de l'ensemble du phytoplancton. Or, les comptages microscopiques ne concernent en général que le micro-phytoplancton. Il est donc nécessaire de prendre en compte des informations supplémentaires qui tiennent compte du nano- et du picophytoplancton, dont l'importance dans la diversité du... |
Tipo: Text |
Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyse CHEMTAX; Projet DYMAPHY. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00361/47258/47223.pdf |
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Artigas, Felipe; Creach, Veronique; Houliez, Emilie; Karlson, Bengt; Lizon, Fabrice; Seppala, Jukka; Wacquet, Guillaume. |
This is a summary of the activities and results of JERICO-NEXT Work Package 3 Innovations in Technology and Methodology, Task 3.1 Automated platform for the observation of phytoplankton diversity in relation to ecosystem services. The aim is to provide an advanced report on the last developments dedicated to the observation of the phytoplankton diversity by applying novel techniques on automated platforms. The work was carried out in close connection with task 4.1 Biodiversity of plankton, harmful algal blooms and eutrophication. The partners involved in these developments are CNRS, SYKE, SMHI, HZG, RWS, VLIZ CEFAS and Ifremer. Subcontractors in WP4, task 4.1 are WHOI, Scanfjord AB, Tomas Rutten b.v., CytoBuoy b.v. and UGent - PAE. The work was carried out... |
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Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00630/74242/73873.pdf |
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Houliez, Emilie; Lefebvre, Sebastien; Lizon, Fabrice; Schmitt, Francois G.. |
This study is the first in situ work comparing rapid light curves (RLC) and non-sequential steady-state light curves (N-SSLC) in their efficiency to characterize phytoplankton photosynthetic activity and acclimation status. Measurements were carried out at two time scales (daily and annual) using the Pulse Amplitude Modulated fluorometry on samples taken in the coastal waters of a macrotidal ecosystem (the Strait of Dover, eastern English Channel). RLC and N-SSLC were compared under a wide range of environmental conditions and phytoplankton composition in order to define the best methodology to accurately capture short and long-term adjustments in the functioning of the photosynthetic apparatus. The relationships between the photosynthetic parameters... |
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Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00395/50596/51484.pdf |
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Artigas, Luis Felipe; Didry, Morgane; Barthelemy, Vanille; Bonato, Simon; Broutin, Mathias; Lizon, Fabrice; Lefebvre, Alain. |
The European Union Water Framework Directive (WFD) refers to phytoplankton as one of the biological quality elements that should be regularly monitored and evaluated thanks to three ecological indices: biomass, abundance and composition. The first two have already been defined, but the third one has not yet been proposed in the English Channel-Atlantic zone. The construction of a composition indice must consider the diversity of all the phytoplankton. As the microscopic counts concern only the micro-phytoplankton, it is necessary to use additional information which concerns the nano- and pico-phytoplankton, of great importance when estimating the phytoplankton diversity. These components of the phytoplankton can be measured by different complementary... |
Tipo: Text |
Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyses pigmentaires; Eastern English Channel; Phytoplankton groups; Scanning Flow Cytometry; Spectral fluorescence; Pigments analysis. |
Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00361/47259/47224.pdf |
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Bonato, Simon; Christaki, Urania; Lefebvre, Alain; Lizon, Fabrice; Thyssen, Melilotus; Artigas, Luis Felipe. |
The distribution of phytoplankton (from pico-to microphytoplankton) was investigated, at single-cell level and at high spatial resolution, during an oceanographic cruise across the eastern English Channel (EEC) between April 27 and 29, 2012. Seawater was continuously collected from surface waters and analysed on board at high frequency (one sample every 10 min), by using a new generation of pulse-shape recording scanning flow cytometer (CytoSense, Cytobuoy©). A Bray-Curtis matrix analysis based on phytoplankton composition allowed the discrimination of 4 communities. Within these communities, abundance, cell size as well as single cell and total red fluorescence of 8 phytoplankton groups were measured. Picoeukaryotes and Synechococcus spp. cells dominated... |
Tipo: Text |
Palavras-chave: Phytoplankton dynamics; High spatial resolution; Flow cytometer; Red fluorescence. |
Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00248/35885/34406.pdf |
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