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Escobedo-fregoso, C.; Arzul, Isabelle; Carrasco, Noelia; Gutierrez-rivera, J. N.; Llera-herrera, Raul; Vazquez-juarez, R.. |
Prevalence of the protozoan Perkinsus spp. in the gills of the pleasure oyster Crassostrea corteziensis from two estuaries in Nayarit, Mexico, was measured. The protozoan was identified by PCR amplification of the internal transcribed spacer (ITS) region of the rDNA of Perkinsus spp. The pathogen was found in 92% of oysters from Boca de Camichín and 77% of oysters from Pozo Chino. ITS sequences characterized from C. corteziensis showed 96–100% similarity to Perkinsus marinus. The most frequent ITS sequence, (GenBank JQ266236) had 100% identity with the ITS locus of P. marinus from New Jersey, Maryland, South Carolina, and Texas, and the second most frequent observed sequence (GenBank JQ266240) was 100% identical to ITS sequences of P. marinus from New... |
Tipo: Text |
Palavras-chave: Crassostrea corteziensis; Perkinsus marinus; Internal transcribed spacer; Non-internal transcribed spacer; Aquatic disease. |
Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00137/24811/22892.pdf |
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Llera-herrera, Raul; Garcia-gasca, Alejandra; Abreu-goodger, Cei; Huvet, Arnaud; Ibarra, Ana M.. |
Despite the great advances in sequencing technologies, genomic and transcriptomic information for marine non-model species with ecological, evolutionary, and economical interest is still scarce. In this work we aimed to identify genes expressed during spermatogenesis in the functional hermaphrodite scallop Nodipecten subnodosus (Mollusca: Bivalvia: Pectinidae), with the purpose of obtaining a panel of genes that would allow for the study of differentially transcribed genes between diploid and triploid scallops in the context of meiotic arrest and reproductive sterility. Because our aim was to isolate genes involved in meiosis and other testis maturation-related processes, we generated suppressive subtractive hybridization libraries of testis vs. inactive... |
Tipo: Text |
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Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00160/27118/25313.pdf |
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