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Isolation and molecular characterization of Bacillus thuringiensis found in soils of the Cerrado region of Brazil, and their toxicity to Aedes aegypti larvae Rev. Bras. entomol.
Lobo,Katiane dos Santos; Soares-da-Silva,Joelma; Silva,Maria Cleoneide da; Tadei,Wanderli Pedro; Polanczyk,Ricardo Antonio; Pinheiro,Valéria Cristina Soares.
ABSTRACT This study investigated the potential of Bacillus thuringiensis isolates obtained in the Cerrado region of the Brazilian state of Maranhão for the biological control of Aedes aegypti larvae. The isolates were obtained from soil samples and the identification of the B. thuringiensis colonies was based on morphological characteristics. Bioassays were run to assess the pathogenicity and toxicity of the different strains of the B. thuringiensis against third-instar larvae of A. aegypti. Protein profiles were obtained by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Polymerase chain reaction assays were used to detect the toxin genes found in the bacterial isolates. Overall, 12 (4.0%) of the 300 isolates obtained from 45 soil samples were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological control; Entomopathogenic bacteria; Vector.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262018000100005
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