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Marchi, Chiara; Andersen, Liselotte Wesley; Loeschcke, V.. |
Several arthropods are known to be highly beneficial to agricultural production. Consequently it is of great relevance to study the importance of land management and land composition for the conservation of beneficial aphid-predator arthropod species in agricultural areas. Therefore our study focusing on the beneficial arthropod Bembidion lampros had two main purposes: I) identifying the physical barriers to the species’ dispersal in the agricultural landscape, and II) assessing the effect of different land management strategies (i.e. use of pesticides and intensiveness) on the dispersal patterns. The study was conducted using genetic analysis (microsatellite markers) applied to samples from two agricultural areas (in Denmark) with different agricultural... |
Tipo: Journal paper |
Palavras-chave: "Organics" in general; Biodiversity and ecosystem services. |
Ano: 2013 |
URL: http://orgprints.org/23289/7/23289.pdf |
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Marchi, C.; Andersen, L.W.; Panitz, F; Holm, L.-E.; Loeschcke, V.. |
454-sequencing of the genome was used to isolate and characterize nuclear 22 microsatellite sequences from the ground beetle Bembidion lampros (Carabidae). A 23 total of 4502 contigs containing di- to penta-nucleotide microsatellite repeats were 24 found (4211 perfect and 289 non-perfect repeats). Primers were developed and tested 25 for 34 microsatellite sequences. Of these 15 were tested on 1140 individual samples 26 amplifying on average 89% of samples and they did not deviate significantly from 27 Hardy-Weinberg and linkage equilibrium. These microsatellite sequences will be useful 28 in studying the effect of agricultural management techniques and various environmental 29 factors on a beneficial agricultural species. |
Tipo: Journal paper |
Palavras-chave: "Organics" in general. |
Ano: 2012 |
URL: http://orgprints.org/21072/1/21072.pdf |
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Ahanchédé, J.E.; Alfaya, F.; Andersen, L.W.; Azam, D.; Bautista, MA.A.M.; Besnard, A.-L.; Bigatti, G.; Bouetard, A.; Coutellec, M.-A.; Ewédjé, E.-E.B.K.; Fuseya, R.; Carcia-Jiménez, R.; Haratian, M.; Hardy, O.J.; Holm, L.-E.; Hoy, C.W.; Koshimizu, E.; Loeschcke, V.; López-Márquez, V.; Machado, C.A.; Machordom, A.; Marchi, C.; Michel, A.P.; Micheneau, C.; Mittapalli, O.; Nagai, T.; Okamoto, N; Pan, Y.; Panitz, F.; Safaie, N.; Sakamoto, T.; Sharifnabi, B.; En-Weitian,; Yu, H.. |
This article documents the addition of 83 microsatellite marker loci and 96 pairs of single-nucleotide polymorphism (NSP) sequencing primers to the Molecular Ecology Resources Database. Loci were developed for the following species: Bembidion lampros, Inimicus japonicus, Lymnaea stagnalis, Panopea abbreviata, Pentadesma butyracea, Sycoscapte rhirticola and Thanatephorus cucumeris (anamorph: Rhizoctonia solani). These loci were cross-tested on the following species: Pentadesma grandiofolia and Pentadesma reyndersii. This article also documents the addition of 96 sequencing primer pairs and 88 allele-specific primers or probes for Plutella zylostella. |
Tipo: Journal paper |
Palavras-chave: "Organics" in general; Biodiversity and ecosystem services. |
Ano: 2013 |
URL: http://orgprints.org/24511/1/24511.pdf |
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