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| Bouvard, Claire; Galy-fauroux, Isabelle; Grelac, Francoise; Carpentier, Wassila; Lokajczyk, Anna; Gandrille, Sophie; Colliec-jouault, Sylvia; Fischer, Anne-marie; Helley, Dominique. |
| Low-molecular-weight fucoidan (LMWF) is a sulfated polysaccharide extracted from brown seaweed that presents antithrombotic and pro-angiogenic properties. However, its mechanism of action is not well-characterized. Here, we studied the effects of LMWF on cell signaling and whole genome expression in human umbilical vein endothelial cells and endothelial colony forming cells. We observed that LMWF and vascular endothelial growth factor had synergistic effects on cell signaling, and more interestingly that LMWF by itself, in the absence of other growth factors, was able to trigger the activation of the PI3K/AKT pathway, which plays a crucial role in angiogenesis and vasculogenesis. We also observed that the effects of LMWF on cell migration were... |
| Tipo: Text |
Palavras-chave: Fucoidan; Angiogenesis; Vasculogenesis; Migration; Signaling; Transcriptomics. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00308/41913/41187.pdf |
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| Zemani, Faouzia; Benisvy, Danielle; Galy Fauroux, Isabelle; Lokajczyk, Anna; Colliec-jouault, Sylvia; Uzan, Georges; Fischer, Anne Marie; Boisson Vidal, Catherine. |
| Endothelial progenitor cell (EPC) transplantation is a potential means of inducing neovascularization in vivo. However, the number of circulating EPC is relatively small, it may thus be necessary to enhance their proangiogenic properties ex vivo prior to injection in vivo. Fucoidan has previously been shown to potentiate in vitro tube formation by mature endothelial cells in the presence of basic fibroblast growth factor (FGF-2). We therefore examined whether fucoidan, alone or combined with FGF-2, could increase EPC proangiogenic potency in vitro. EPC exposure to 10 mu g/ml fucoidan induced a proangiogenic phenotype, including cell proliferation (p < 0.01) and migration (p < 0.01); moreover, differentiation into vascular cords occurred in the... |
| Tipo: Text |
Palavras-chave: Fucoidan; FGF 2; Endothelial progenitor cells; Angiogenesis; Alpha 6. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-679.pdf |
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