| The present study investigated the possible use of emulsions as an artificial lipid supplement to live algae for seed of the Manila clam <i>Tapes philippinarum</i>. The uptake and assimilation of an emulsion, rich in docosahexaenoic acid (DHA, 22:6n-3) and eicosapentaenoic acid (EPA, 20:5n-3) and fed at concentrations of 20 and 40% of the algal dry weight, were verified analytically by fatty acid analysis of the animals and their diets. Dietary requirements for n-3 highly unsaturated fatty acids (HUFA) were examined by supplementing <i>Dunaliella tertiolecta</i>, which contains no polyunsaturated fatty acids longer than 18:3n-3 and <i>Tetraselmis suecica</i>, which contains EPA but only trace amounts of DHA. An algal... |
