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| Zheng,Lei; Yang,Lixia; Zhao,Xin; Long,Niya; Li,Peifan; Wang,Yiming. |
| This aim of this study was to assess the molecular mechanism of osteoporosis in schizophrenia patients with risperidone use. Here, we investigated the effects of risperidone on cellular proliferation and apoptosis of a preosteoblast cell line, MC3T3-E1. Cell viability and apoptotic rate of MC3T3-E1 were detected by cell counting kit-8 and flow cytometry at a serial dose of risperidone and at different time points, respectively. Bone transformation relevant gene serum osteocalcin (BGP), collagen 1, tumor necrosis factor-α (TNF-α), osteoprotegerin (OPG), and receptor activator of nuclear factor-κB ligand (RANKL) mRNA levels were determined by real-time PCR (qPCR). Their protein expression patterns were evaluated using western blot. The results revealed that... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Risperidone; MC3T3-E1 cells; Collagen 1; Tumor necrosis factor-α; Receptor activator of nuclear factor-κB ligand. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000300605 |
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