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Registros recuperados: 3
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Functional differences between two morphologically distinct cell subpopulations within a human colorectal carcinoma cell line BJMBR
Solimene,A.C.C.; Carneiro,C.R.W.; Melati,I.; Lopes,J.D..
The LISP-I human colorectal adenocarcinoma cell line was isolated from a hepatic metastasis at the Ludwig Institute, São Paulo, SP, Brazil. The objective of the present study was to isolate morphologically different subpopulations within the LISP-I cell line, and characterize some of their behavioral aspects such as adhesion to and migration towards extracellular matrix components, expression of intercellular adhesion molecules and tumorigenicity in vitro. Once isolated, the subpopulations were submitted to adhesion and migration assays on laminin and fibronectin (crucial proteins to invasion and metastasis), as well as to anchorage-independent growth. Two morphologically different subpopulations were isolated: LISP-A10 and LISP-E11. LISP-A10 presents a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Carcinoembryonic antigen; CEA; Colorectal carcinoma cell line; Laminin; Fibronectin; Desmoglein; Metastasis.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500014
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The low efficiency of dendritic cells and macrophages from mice susceptible to Paracoccidioides brasiliensis in inducing a Th1 response BJMBR
Almeida,S.R.; Lopes,J.D..
In the present study we evaluated T cell proliferation and Th lymphokine patterns in response to gp43 from Paracoccidioides brasiliensis presented by isolated dendritic cells from susceptible and resistant mice. T cell proliferation assays showed that dendritic cells from susceptible mice were less efficient than those from resistant mice. The pattern of T cell lymphokines stimulated by dendritic cells was always Th1, although the levels of IL-2 and IFN-gamma were lower in T cell cultures from susceptible mice. To determie whether different antigen-presenting cells such as macrophages and dendritic cells stimulated different concentrations of Th1 lymphokines, the production of IFN-gamma and IL-2 was measured. It was observed that dendritic cells were more...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dendritic cells; Paracoccidioidomycosis; Paracoccidioides brasiliensis; Th1/Th2.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000400014
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Laminin concentration in ascites of patients with hepatic cirrhosis and peritoneal carcinomatosis BJMBR
Catarino,R.M.; Lopes,J.D.; Forones,N.M.; Parise,E.R..
Laminin levels in ascitic fluid have been proposed as a marker for neoplastic ascites. We compared the concentration of laminin in serum and in ascitic fluid from patients with hepatic cirrhosis and peritoneal carcinomatosis and assessed the diagnostic value of serum laminin levels in differentiating neoplastic from benign ascites. Laminin concentrations were determined by ELISA with antibodies against laminin extracted from the human placenta, in patients with ascites due to peritoneal carcinomatosis (N = 20) and hepatic cirrhosis (N = 33). Patients with infected or hemorrhagic ascites were excluded. The receiver operating characteristic curve was used to determine the sensitivity and specificity of serum laminin for the diagnosis of neoplastic ascites....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ascites laminin; Serum laminin; Peritoneal carcinomatosis; Cirrhosis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200016
Registros recuperados: 3
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