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Surface response methodology for the optimization of lipase production under submerged fermentation by filamentous fungi BJM
Colla,Luciane Maria; Primaz,Andreiza Lazzarotto; Benedetti,Silvia; Loss,Raquel Aparecida; Lima,Marieli de; Reinehr,Christian Oliveira; Bertolin,Telma Elita; Costa,Jorge Alberto Vieira.
Abstract A Plackett–Burman Factorial Design of 16 experiments was conducted to assess the influence of nine factors on the production of lipases by filamentous fungi. The factors investigated were bran type (used as the main carbon source), nitrogen source, nitrogen source concentration, inducer, inducer concentration, fungal strain (Aspergillus niger or Aspergillus flavus were selected as good lipase producers via submerged fermentation), pH and agitation. The concentration of the yeast extract and soybean oil and the pH had a significant effect (p < 0.05) on lipase production and were consecutively studied through a Full Factorial Design 23, with the concentration of yeast extract and pH being significant (p < 0.05). These variables were optimized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural residues; Lipase; Optimization; Submerged bioprocess.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200461
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