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| Targon,Maria Luísa P. Natividade; Takita,Marco Aurélio; Amaral,Alexandre M. do; Souza,Alessandra A. de; Locali-Fabris,Eliane Cristina; Dorta,Sílvia de Oliveira; Borges,Kleber Martins; Souza,Juliana Mendonça de; Rodrigues,Carolina Munari; Lucheta,Adriano Reis; Freitas-Astúa,Juliana; Machado,Marcos Antonio. |
| In order to obtain a better understanding of what is citrus, 33 cDNA libraries were constructed from different citrus species and genera. Total RNA was extracted from fruits, leaves, flowers, bark, seeds and roots, and subjected or not to different biotic and abiotic stresses (pathogens and drought) and at several developmental stages. To identify putative promoter sequences, as well as molecular markers that could be useful for breeding programs, one shotgun library was prepared from sweet orange (Citrus sinensis var. Olimpia). In addition, EST libraries were also constructed for a citrus pathogen, the oomycete Phythophthora parasitica in either virulent or avirulent form. A total of 286,559 cDNA clones from citrus were sequenced from their 5 end,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: ESTs; Shotgun; Citrus; Phytopathogen. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000500030 |
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